FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902974
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG50260.5566051735708891No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAACACCCA47680.52803292232113No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC41250.45682378451649774No Hit
GACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAACACCCAACGGCCACTC40400.4474104459264608No Hit
GATTATTACTGTGCAGCATGGGATGACAACCTGAGTGGCCGTTGGGTGTTCGGCGCAGGGACCAAGCTGACCGTC38560.4270333365080279No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC36250.4014512045751041No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35640.3946957498222541No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC32450.3593680438196448No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT30200.3344503828460177No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29920.33134951836929966No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG29520.32691971197398817No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28170.3119691153898119No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27780.3076500541543832No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26980.2987904413637602No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26000.287937415695247No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGTCAGAGGGTCAC24830.27498023198896093No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23870.2643486966402133No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21360.23655166150963375No Hit
ACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAACACCCAACGGCCACTCA21340.23633017118986815No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTG20340.2252556552015894No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20320.22503416488182387No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20320.22503416488182387No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC20230.22403745844287878No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18380.20354960386456308No Hit
GTTTGGGGGCCGTTCCTGGGAACTGCTGATACCAGTATACAAAATTACTT18110.20055948454772785No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17740.1964619136320647No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT17680.195797442672768No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17120.1895957137193319No Hit
CCCAACGGCCACTCAGGTTGTCATCCCATGCTGCACAGTAATAATCAGCC16970.18793453632109008No Hit
CCCCTGAGGGCCGCCGACTATTCCTATAGATGAGCAGTTTGGGGGCCGTTCCTGGGAACTGCTGATACCAGTATA16820.18627335892284827No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16710.18505516216413762No Hit
GCATGGGATGACAACCTGAGTGGCCGTTGGGTGTTCGGCGCAGGGACCAA16650.1843906912048409No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATG16490.1826187686467163No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15730.17420213649562447No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC15700.1738699010159761No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15100.16722519142300885No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14940.16545326886488426No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14850.16445656242593917No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14840.16434581726605638No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14700.1627953850276974No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14700.1627953850276974No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14270.1580333431527375No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14050.15559694963531617No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14040.1554862044754334No Hit
GTGCAGCATGGGATGACAACCTGAGTGGCCGTTGGGTGTTCGGCGCAGGGACCAAGCTGACCGTCCTAGGTCAGC13980.15482173351613668No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAACAC13970.1547109883562539No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13960.1546002431963711No Hit
TCTATAGGAATAGTCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC13820.15304981095801207No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13690.15161012387953585No Hit
GCCCCCAAACTGCTCATCTATAGGAATAGTCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13610.15072416260047355No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13560.15017043680105963No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12880.14263976592903008No Hit
GCTTGGTCCCTGCGCCGAACACCCAACGGCCACTCAGGTTGTCATCCCATGCTGCACAGTAATAATCAGCCTCAT12730.14097858853078826No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG12640.13998188209184317No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG12580.13931741113254645No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12080.13378015313840708No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11860.13134375962098577No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC11860.13134375962098577No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11640.12890736610356443No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG11630.12879662094368166No Hit
GTCCCTGCGCCGAACACCCAACGGCCACTCAGGTTGTCATCCCATGCTGC11380.126027991946612No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAACCTGA11050.12237340167047998No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCCG10940.12115520491176933No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10890.12060147911235539No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10870.12037998879258982No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10670.11816508559493408No Hit
CCCTCAGCGTCTGGGACCCCCGGTCAGAGGGTCACCATCTCTTGTTCTGG10590.11727912431587179No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG10550.11683614367634061No Hit
GGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTGCTCATCTATAGGAATAGTCGGCGGCCCTCAGGGGTCC10540.11672539851645784No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10480.1160609275571611No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10410.11528571143798161No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG10370.11484273079845045No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10360.11473198563856768No Hit
GTCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCGCCAACATCGGAAGT10320.1142890049990365No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10310.11417825983915374No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10300.11406751467927094No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC10180.1127385727606775No Hit
GACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAACACCCAACGGCCACTCAGGTTGTCATCCCATGCTGCACAGT10040.11118814052231847No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10040.11118814052231847No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG9980.11052366956302173No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9970.11041292440313896No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGTCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG9610.10642609864735862No Hit
GCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG9520.10542939220841353No Hit
CTCTTGTTCTGGAAGCAGCGCCAACATCGGAAGTAATTTTGTATACTGGTATCAGCAGTTCCCAGGAACGGCCCC9480.10498641156888237No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9410.10421119544970288No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA9340.10343597933052337No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAACACCCAACGGCCA9220.10210703741192992No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA9080.10055660517357089No Hit
TTACTGTGCAGCATGGGATGACAACCTGAGTGGCCGTTGGGTGTTCGGCG9050.10022436969392252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)