FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355534
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA200.00757612351.36349524
GTATCAA10850.040.69491
CCTATAC451.04791005E-438.0309753
TATCAAC12400.035.6080362
CAACGCA13050.033.5721745
TCAACGC13100.033.4440354
AACGCAG13300.032.945756
GGTATCA4300.032.6358871
ATCAACG13450.032.5737463
TATACAC1500.031.946023
ACGAGAC1208.367351E-1131.38438822
ATTGGTA450.004800838430.424783
CAGAGTA14450.030.08686610
ACGCAGA14600.030.0122287
AGAGTAC14700.029.57518611
CGCAGAG14900.029.4079558
CTTATAC1302.1464075E-1028.9620511
GCAGAGT15150.028.9226729
TAGCGGC755.031555E-528.42593464
CGAGACG1254.48199E-927.39000923
Produced by FastQC (version 0.11.5)