FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550180
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGT150.002462621568.2259613
GCGATTA200.00705267852.3057245
ACCTATC200.007473759451.54426634
GTATTCT200.007528019651.45005432
AGAGTCG901.382432E-1037.9033111
ATAGCCG451.07019754E-437.89986816
GTATCAA24050.035.5990831
TCGAGTA501.9886984E-434.1098822
TCGTATG851.1712655E-732.5801440
GGTATCA10900.032.5452041
TATACAC1650.031.0117993
CTTATAC1550.030.8089241
TCCTATC1151.7898856E-929.85613333
AACGCAG28900.029.5094996
CAATACA704.155204E-529.2396974
TTATACA1750.029.2370412
CAACGCA29550.029.0912725
TATCAAC29700.029.0565662
TCAACGC30100.028.5597044
TAGTATC854.828378E-628.0930424
Produced by FastQC (version 0.11.5)