FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3129378
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTCCAGGGAGA100540.3212779025097No Hit
CTCCTGCACTGTCTCTGGTGACTCCACCAACAGTCCTATTTACTACTGGG76950.2458955102259938No Hit
GTCTATTACTGTGCGAGGGGCAGTCCTTCCTTCTTCGACCTCTATAGTAAGGAGTACTACTTTGACTACTGGGGC73330.2343277162426527No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC71230.22761711752303493No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT69000.2204911007874408No Hit
GTCTCTGGTGACTCCACCAACAGTCCTATTTACTACTGGGGCTGGATCCG66170.21144777013195593No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT66110.21125603873996687No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC57810.1847331961814776No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT57310.1831354345815686No Hit
CTATAGTAAGGAGTACTACTTTGACTACTGGGGCCAGGGAATCCTGGTCACCGTCGCCTCAGCCTCCACCAAGGG55210.17642483586195085No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG55180.1763289701659563No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG50030.15987202568689368No Hit
TTACTATAGAGGTCGAAGAAGGAAGGACTGCCCCTCGCACAGTAATAGAC46750.1493907095914907No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45990.14696211195962902No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA44890.14344703643982926No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA43880.1402195580080131No Hit
AGCTTACACTGGTACCAGCAGAAACCACATCAGTCTCCCAAGCTCCTCAT42290.1351386761203025No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA41910.13392437730437168No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT40510.12945064482462648No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC40010.1278528832247175No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC38180.12200507576905059No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC36970.11813849269727084No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA36650.11711592527332909No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT35500.1134410735935384No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCTCCTGCA35310.11283392418557298No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG35050.11200308815362031No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA34830.11130007304966036No Hit
CTATTATAGTGGGACCACCTTCTACAACCCGGCCCTCAAGAGTCGAGTCA33190.10605941500195885No Hit
GTATTACTGTCATCAGAGTAATAGTTTACCTCACACTTTTGGCCAGGGGA31430.10043529417027922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAT29900.049.67448870
GGTATCA23150.040.5926251
GTATCAA53600.039.1100161
GTGGTAT8950.036.1526641
TATCAAC60250.034.908132
TGGTATC9200.034.7966582
GTTCGGA30200.033.7332969
ATCAACG63550.033.0423243
TCAACGC63600.032.9632764
CAACGCA65050.032.2819445
AACGCAG66250.031.9050646
AGAGTAC75450.028.05675711
CGCAGAG76650.027.5748088
GCAGAGT78100.027.1505789
TGTTCGG34350.026.93456368
ACGCAGA80450.026.2295447
CGTGTGT34200.025.60743764
ACGTGTG33950.025.55251363
TTCGGGG42550.025.33030970
GTGTTCG35350.025.2926367
Produced by FastQC (version 0.11.5)