FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3129378
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTCCAGGGAGA97400.31124395966227153No Hit
CTCCTGCACTGTCTCTGGTGACTCCACCAACAGTCCTATTTACTACTGGG72940.23308146219472367No Hit
GTCTATTACTGTGCGAGGGGCAGTCCTTCCTTCTTCGACCTCTATAGTAAGGAGTACTACTTTGACTACTGGGGC71990.23004571515489658No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT70590.22557198267515144No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT66000.21090453118798685No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC64120.20489694757232904No Hit
GTCTCTGGTGACTCCACCAACAGTCCTATTTACTACTGGGGCTGGATCCG62700.20035930462858753No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT62120.1985059011726931No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG53060.16955446098234217No Hit
CTATAGTAAGGAGTACTACTTTGACTACTGGGGCCAGGGAATCCTGGTCACCGTCGCCTCAGCCTCCACCAAGGG52890.16901122203837313No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC52610.1681164755424241No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46270.14785685845557808No Hit
TTACTATAGAGGTCGAAGAAGGAAGGACTGCCCCTCGCACAGTAATAGAC46110.1473455747436072No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG45610.1457478131436982No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA44940.14360681259982017No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA41500.13261421279244628No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40950.13085667503254642No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC39530.12631903208880488No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA39260.12545624082485402No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT39120.1250088675768795No Hit
AGCTTACACTGGTACCAGCAGAAACCACATCAGTCTCCCAAGCTCCTCAT38890.12427389724092136No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT36250.11583771599340188No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCTCCTGCA35640.1138884468415129No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC34740.11101247596167674No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC34410.10995795330573681No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT34170.10919102773778047No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT34060.1088395201858005No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT33780.10794477368985148No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA33470.1069541614979079No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT33000.10545226559399343No Hit
CTATTATAGTGGGACCACCTTCTACAACCCGGCCCTCAAGAGTCGAGTCA32710.10452556386604624No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA31980.1021928319301791No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA31760.10148981682621915No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA31590.10094657788225009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAT28850.047.9759470
GGTATCA24600.039.8879551
GTGGTAT8900.039.4586451
GTATCAA58000.039.35661
TGGTATC9650.035.6498262
TATCAAC65950.034.7411462
ATCAACG68850.033.209283
TCAACGC68850.033.2066274
CAACGCA71300.032.1138045
AACGCAG72400.031.9108076
GTTCGGA28300.031.5379969
CGCAGAG80050.028.9900888
AGAGTAC79900.028.78633911
GCAGAGT85000.027.2613939
ACGCAGA87400.026.4734617
TGTTCGG32350.024.8128268
TTCGGGG36950.024.54829870
TTGGACG33100.023.99501459
ACGTGTG32350.023.78663363
GTGTTCG32900.023.75372367
Produced by FastQC (version 0.11.5)