FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548916
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAT305.887961E-446.64837653
GTATCAA13550.038.7093121
GGTATCA5650.033.3716431
TATCAAC16300.031.9711952
ATCAACG16750.031.5216393
TCAACGC16550.031.2810864
CAACGCA17200.030.4976165
AACGCAG17300.030.1231526
AGAGTAC19300.026.81660711
ACGCAGA19550.026.6490047
ACCGTTC658.982613E-426.3681958
CAGAGTA19900.026.00806410
ACGGGAC1209.063842E-825.70430616
CGCAGAG20300.025.6667778
GCAGAGT20600.025.4570679
ATAATAC700.001382426624.4892163
TCTGTCG1001.6415292E-523.9950588
AGCTAGC750.001866955823.25714950
GTATAAT850.004271550623.08613870
GGACCTA902.2162953E-422.8566026
Produced by FastQC (version 0.11.5)