FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548916
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9500.17306837476043693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG200.007533940551.4398351
GTATCAA15050.037.14151
GGTATCA6650.035.582441
TATCAAC16900.033.0485762
TCAACGC17550.032.1915634
CGTTAAC652.2870245E-531.91148239
ATCAACG18000.031.3896333
CAACGCA18050.031.2998315
AACGCAG18050.031.2998316
GGTCTAT450.0047584930.4828641
TGCGTTA703.8249687E-529.59670437
AATGCGT703.8635022E-529.55326735
ACGCAAT604.7998567E-429.35157459
AGAGTAC19250.029.17080311
ACGCAGA19500.028.9724087
CGCAGAG19550.028.7231668
CAGAGTA19550.028.72316610
GCGTTTA605.64256E-428.55163825
GCAGAGT19950.027.9756349
GCGTTAA756.098968E-527.63882438
Produced by FastQC (version 0.11.5)