FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146600
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34070.29713936856794No Hit
GAGTAACCCTCACTTGCCGGGCAAGTGAGAGCATAGACACCTATTTAAATTGGTATCAGCAGAAGCCAGGGAAAG24340.21227978370835515No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22920.1998953427524856No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21210.184981684981685No Hit
GTTGGAGACAGAGTAACCCTCACTTGCCGGGCAAGTGAGAGCATAGACACCTATTTAAATTGGTATCAGCAGAAG20740.18088260945403803No Hit
GAGTTACAGTACCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20540.17913832199546487No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19730.1720739577882435No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19320.1684981684981685No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19050.1661433804290947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18580.16204430490144778No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG18480.16117216117216118No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18440.16082330368044653No Hit
CTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCTGGTCGCAGACTGCTGATGGTGAGAGTGAAATCTGTC17270.15061922204779346No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16320.1423338566195709No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCTGGTCGCAGACTGCTGATGGTGAGAGTGAAAT16150.1408512122797837No Hit
GTCTCTGGTGGCTCCATCAGTGATTATTACTGGAGCTGGATCCGGCAGTC15400.1343101343101343No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15020.13099598813884528No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14830.12933891505320078No Hit
GTATATTACTGTGCGAGGGCGGTCGAATATAGCAGTGGCTGGCGCTACTACTTTGACCACTGGGGCCAGGGAGCC14810.12916448630734345No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14780.1289028431885575No Hit
GTTACAGTACCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA14470.12619919762776904No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14240.12419326705040991No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGACCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA14130.12323390894819466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13950.12166405023547881No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13900.1212279783708355No Hit
GGCCAAAAGTGTGAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTCGCA13680.11930926216640503No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13550.11817547531833246No Hit
GATCTATGGTGCATCCAGTCTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGAATCTGGGACAGATTTCA13360.11651840223268793No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13150.11468690040118612No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12810.11172161172161173No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12610.10997732426303855No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12370.10788417931275074No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12230.10666317809174951No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11950.10422117564974707No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGAGGGGTA11810.10300017442874584No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11650.10160474446188732No Hit
GTAGTAAGTCGCAAAATCTTCTGGTCGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATTCACTGCCA11570.10090702947845806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA200.00658723553.2203365
GTATCAA29750.042.4353451
TATCAAC32950.038.2100452
GTGGTAT3050.037.1176951
ATCAACG34050.036.7757533
TCAACGC34200.036.5157364
CAACGCA34650.036.0430765
AACGCAG34900.035.8831986
CGCAGAG38300.032.6948938
AGAGTAC38700.032.17685711
ACGCAGA40550.030.9653557
ACGCGGA703.9219616E-529.49239232
AGTACGG26500.028.21887213
GAGTACG26700.028.00749412
CGACGTC1555.4569682E-1126.62198630
CTCGACG1555.4569682E-1126.5975728
TCGACGT1608.185452E-1125.77877829
AGAGTCG809.938874E-525.72818811
CGTGTCT6450.024.69349564
GTTAAGC851.3244196E-424.66328854
Produced by FastQC (version 0.11.5)