FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146600
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32810.286150357578929No Hit
GAGTAACCCTCACTTGCCGGGCAAGTGAGAGCATAGACACCTATTTAAATTGGTATCAGCAGAAGCCAGGGAAAG23940.20879120879120883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22640.19745334031048317No Hit
GTTGGAGACAGAGTAACCCTCACTTGCCGGGCAAGTGAGAGCATAGACACCTATTTAAATTGGTATCAGCAGAAG22440.19570905285190998No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21140.18437118437118438No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19990.17434153148438863No Hit
GAGTTACAGTACCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19890.17346938775510204No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19740.17216117216117216No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19410.16928309785452644No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19030.1659689516832374No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18880.1646607360893075No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG18460.16099773242630386No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCTGGTCGCAGACTGCTGATGGTGAGAGTGAAAT16360.14268271411128555No Hit
CTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCTGGTCGCAGACTGCTGATGGTGAGAGTGAAATCTGTC16280.14198499912785625No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15350.13387406244549102No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGACCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA15290.13335077620791905No Hit
GTCTCTGGTGGCTCCATCAGTGATTATTACTGGAGCTGGATCCGGCAGTC15200.13256584685156114No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15190.13247863247863248No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14820.1292517006802721No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14730.12846677132391418No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14240.12419326705040991No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14110.12305948020233735No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14100.12297226582940869No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13840.12070469213326357No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13700.11948369091226234No Hit
GTATATTACTGTGCGAGGGCGGTCGAATATAGCAGTGGCTGGCGCTACTACTTTGACCACTGGGGCCAGGGAGCC13400.1168672597244026No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13270.11573347287633003No Hit
GGCCAAAAGTGTGAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTCGCA13160.11477411477411477No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12960.1130298273155416No Hit
GATCTATGGTGCATCCAGTCTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGAATCTGGGACAGATTTCA12770.11137275422989708No Hit
GTTACAGTACCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12460.10866910866910867No Hit
GTAGTAAGTCGCAAAATCTTCTGGTCGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATTCACTGCCA12320.10744810744810745No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11990.10457003314146172No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11960.10430839002267572No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11930.10404674690388975No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11890.10369788941217513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG300.002615398767.19852470
GTGGTAT4150.042.9805451
GTATCAA30150.042.3225981
TATCAAC33450.037.9190062
TCAACGC35600.035.6165244
ATCAACG35600.035.4240043
GTACGTA400.002453970734.8989451
CAACGCA36400.034.8337445
AACGCAG36950.034.4079866
AGAGTAC39950.031.73837911
CGCAGAG40550.031.268768
ACGCAGA42050.030.1533457
AGTACGG28450.027.10073913
GAGTACG28700.026.86466812
AGTACTT18950.026.0395913
TGGATTG8900.025.67134570
GAGTACT19550.025.24042112
TTCGGGG6800.024.7053470
TAACCGT851.5106419E-424.189797
GTACGGG34100.022.8114214
Produced by FastQC (version 0.11.5)