FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969668
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTG18870.19460268875532657No Hit
TCTCTGGGCTGCAGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT17140.1767615307507312No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTGCCTGCATATGAGCTG16780.1730489198364801No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC15870.16366426446990104No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG15720.16211734325562976No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13700.14128547090344323No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13400.13819162847490069No Hit
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAACA13390.13808850039394927No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCACCATCTCCTG13200.1361290668558723No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13040.13447901756064962No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12560.12952886967498153No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11710.12076298279411098No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11570.11931918966079111No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11430.11787539652747127No Hit
CTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCT10930.11271899247990035No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGC10710.11045017469896914No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10230.10550002681330105No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10170.10488125832759253No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT10030.10343746519427269No Hit
GCCCTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTA9990.10302495287046701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG150.002287149269.51375647
CCTAACG200.007446533551.59490621
GGTATCA7350.041.6524541
GTATCAA18550.038.0143781
CGCTAAT952.2191671E-1036.29304532
GTGGTAT3400.033.3866581
TATCAAC20750.033.320842
ATCAACG21450.032.714543
CTCGATT800.003415818632.61857270
TCAACGC21550.032.0838664
TGGTATC3550.031.9759562
TCGTATG1101.022272E-931.4627240
CAACGCA22100.031.4442925
AACGCAG23000.030.064296
CTCGTAT1052.277011E-829.64177939
TCGCTAA1202.7175702E-928.71566831
CGAATCG500.00784844227.5172821
TGTTCGG8350.027.3449170
CCCTATA4200.027.0272942
GCTAATC1155.637594E-826.99983833
Produced by FastQC (version 0.11.5)