FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969668
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTG17340.17882409236975955No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC16150.16655185073654075No Hit
TCTCTGGGCTGCAGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT15190.15665155496520458No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14800.15262955980809928No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTGCCTGCATATGAGCTG14520.14974197354145957No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCACCATCTCCTG14490.1494325892986053No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG14130.14571997838435424No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13580.14004793393202622No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12840.13241645594162127No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12590.1298382539178358No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11790.12158800744172232No Hit
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAACA11490.11849416501317978No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGC10870.11210022399419181No Hit
CTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCT10800.1113783274275319No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10660.10993453429421204No Hit
GCCCTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTA10430.10756258843232942No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10340.10663443570376664No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9790.10096239125143863No Hit
ATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCTCAGCTTTGACTACTGGGGCCAGGGAAG9710.1001373666038273No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9700.10003423852287588No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT9700.10003423852287588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19750.037.798061
GGTATCA8450.037.4548451
GTGGTAT3350.036.9687351
TATCAAC21850.033.9850462
TCAACGC22750.032.783244
CAACGCA23000.032.277475
ATCAACG23100.032.137743
AACGCAG23750.031.4028956
TGGTATC4000.030.0810932
TGTTCGG10700.029.68058270
CGCAGAG25400.029.3629428
AGAGTAC25500.028.97673611
GCAGAGT26450.028.457199
CAGAGTA26600.028.1675110
AATAGCG755.3984986E-528.14143262
TCTAGAC658.8497513E-426.4380973
TCTGGAG10050.025.6751870
TTCGGGG7600.025.24639370
AGTACGG23150.023.60341813
ACGCAGA32950.023.2607127
Produced by FastQC (version 0.11.5)