FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873622
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC61050.6988148192238749No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47800.5471473932661952No Hit
GTATAATAGTTATTCCTTACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT46470.5319234176794999No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39540.45259849225408705No Hit
GAGTATTAGTGACTGGTTGGCCTGGTATCAGCAGAGGCCAGGAAAACCCCCTAAGCTCCTGATCTATAAGGCGT39100.44756198905247346No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37230.426156850445616No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36000.4120775346774692No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33040.37819560404843283No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30280.3466029930564935No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30260.3463740610927838No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28710.3286318339052817No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28570.32702931015931375No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27890.31924562339318374No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24990.28605048865527655No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG24590.28147184938108244No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24190.27689321010688833No Hit
GGTATCAGCAGAGGCCAGGAAAACCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT24030.2750617543972107No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23900.2735736966330976No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23620.27036864914116177No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA22650.25926544890124104No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22600.2586931189919668No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22370.25606040140930514No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA22350.2558314694455954No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21650.24781885071575577No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGACTGGTTGGCCTGGTATCAGCAGAGG21540.2465597249153524No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21350.24438487126011021No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20740.2374024463669642No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20440.23396846691131862No Hit
GGCCAGGAAAACCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAA20250.2317936132560764No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20080.22984769156454396No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19940.228245167818576No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19910.22790176987301144No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19840.22710050800002746No Hit
GTATCAGCAGAGGCCAGGAAAACCCCCTAAGCTCCTGATCTATAAGGCGT18250.2089004168851059No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17900.20489410752018605No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17620.2016890600282502No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17620.2016890600282502No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA17110.1958512949536527No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16880.1932185773709911No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16380.1874952782782485No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16240.18589275453228055No Hit
CCTTATAGATCAGGAGCTTAGGGGGTTTTCCTGGCCTCTGCTGATACCAGGCCAACCAGTCACTAATACTCTGA15790.1807417853488122No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA15780.18062731936695733No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC15770.18051285338510248No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15520.17765120383873118No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15390.17616314607461808No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15370.17593421411090837No Hit
GCTGATACCAGGCCAACCAGTCACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG15250.17456062232865016No Hit
TTCCTGGCCTCTGCTGATACCAGGCCAACCAGTCACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACT14800.16940965314518178No Hit
CAGTATAATAGTTATTCCTTACTCACTTTCGGCGGAGGGACCAAGGTGGA14780.16918072118147207No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14470.16563227574397163No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTATTCCTTACTCA14200.16254169423389064No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14180.16231276227018093No Hit
CTGCTGATACCAGGCCAACCAGTCACTAATACTCTGACTGGCCCGGCAAG13950.1596800446875193No Hit
GTATTAGTGACTGGTTGGCCTGGTATCAGCAGAGGCCAGGAAAACCCCCT13900.15910771477824506No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13800.1579630549596965No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13530.15487247344961552No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13420.1536133476492121No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13350.15281208577622815No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13320.1524686878306636No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13280.1520108239032442No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13140.15040830015727624No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT13060.14949257230243743No Hit
ATCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGGTTTTCCTGGCCTCTG13000.14880577641130832No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12860.1472032526653404No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGA12790.1464019907923564No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12720.14560072891937245No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12250.14022082777219438No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12020.13758811018953276No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11930.1365579163528391No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG11790.13495539260687117No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11740.1343830626975969No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11660.13346733484275808No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11480.13140694716937074No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11460.13117801520566102No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11360.1300333553871125No Hit
TTATAGATCAGGAGCTTAGGGGGTTTTCCTGGCCTCTGCTGATACCAGGC11330.12968995744154793No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11110.1271717058407412No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11060.12659937593146692No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11040.1263704439677572No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11030.12625597798590235No Hit
TAGTTATTCCTTACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT10740.12293646451211164No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC10690.12236413460283739No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10650.12190627067541797No Hit
CATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG10600.1213339407661437No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCACTTTCTAAACTAGA10510.12030374692945003No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10500.12018928094759518No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10470.11984588300203063No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC10320.11812889327420784No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC10120.1158395736371108No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9980.11423704989114285No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9920.11355025400001373No Hit
GTCAGAGTATTAGTGACTGGTTGGCCTGGTATCAGCAGAGGCCAGGAAAACCCCCTAAGCTCCTGATCTATAAGG9870.11297792409073948No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9720.11126093436291669No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9660.11057413847178757No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9410.10771248892541625No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGTAAGGAATAACTATTATACTGTTG9400.1075980229435614No Hit
GTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCACTTTCTAAACT9300.10645336312501288No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT9260.10599549919759346No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9170.10496530536089979No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGG9170.10496530536089979No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGGTTTTCCTGGCC9150.1047363733971901No Hit
GATCAGGAGCTTAGGGGGTTTTCCTGGCCTCTGCTGATACCAGGCCAACC9130.10450744143348038No Hit
ATTAGTGACTGGTTGGCCTGGTATCAGCAGAGGCCAGGAAAACCCCCTAA9100.10416404348791582No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9000.1030193836693673No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCCTTACTCACTTTCGGCGG8940.10233258777823817No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG8890.10176025786896392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAA150.001659543575.3669768
ACCCGTT150.00235657668.9909637
AAACGAG250.00763589851.2667369
GTATCAA15100.043.1738361
CGTATGC1050.039.54402541
TCGTATG1050.039.5234940
GTGGTAT1850.038.948571
GTATGGG451.03413906E-438.1242681
TATCAAC18100.036.0179522
ATCAACG18300.035.6243133
TCAACGC18500.035.239184
GTATGCC1300.034.6230142
ATCTCGT1003.8198777E-1034.49548337
AACGCAG18950.034.4043436
CATAGAC400.002640455334.38072230
CAACGCA19000.034.3138055
CTTATAC1400.034.311841
TATACAC1400.034.311843
ATAAACG400.002666551934.3118364
ATCTCCG1400.034.3079110
Produced by FastQC (version 0.11.5)