FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166673
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC88750.7607101561448666No Hit
GGTCTATACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT69650.5969967591604503No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45970.3940264324279383No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA40790.34962667345520126No Hit
GTGATGGAAACACCTACTTGCATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT30630.26254143191794105No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29700.2545700466197469No Hit
GTCTATACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA25980.22268450542697055No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25560.21908452496972158No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25500.21857024204725745No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25500.21857024204725745No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25000.214284551026723No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23600.2022846161692265No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22660.19422751705062172No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21770.18659898703407038No Hit
GTAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATCCATTGAGTTCTTGGCGTTGTCTCTGGA20450.1752847627398594No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA19870.17031336115603946No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC19540.1674848050824867No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19180.16439910754770187No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18740.16062769944963157No Hit
GTGTACTACTGTGCGAGGGCCGATACATTTGGAACTGTGGACGTCTGGGG18200.15599915314745433No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18050.154713445841294No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG17790.1524848865106161No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16880.14468492885324336No Hit
CTCGTACACAGTGATGGAAACACCTACTTGCATTGGTTTCAGCAGAGGCC16670.1428849386246189No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC16660.1427992248042082No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGCTGTGATGACTCAGT15580.13354213219985378No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA15240.13062786230589035No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14930.127970733873159No Hit
ATACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT14210.12179933880358934No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGCTGTGATGACTCAGTCTCCACTCTCC14210.12179933880358934No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14030.12025649003619694No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13830.11854221362798316No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13460.11537080227278768No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13460.11537080227278768No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13300.11399938114621663No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13160.11279938766046699No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGCTGTGATGACTCAGTCTCCACT12970.1111708250726639No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12540.10748513079500426No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA12490.1070565616929508No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12420.10645656495007598No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12350.10585656820720116No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12260.10508514382350496No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12220.1047422885418622No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12150.10414229179898737No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12010.10294229831323773No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11670.1000280284192743No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA11670.1000280284192743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGTG24100.052.840670
GGTATCA12050.042.1262251
GTATCAA31100.042.018681
TGGTATC3850.041.8711552
GTGGTAT4500.037.347491
CTTATAC2900.036.664141
TATCAAC35950.036.1591452
AATCAGT24350.034.9742869
TATCGGG1102.910383E-1134.48198736
TATACAC3100.034.2987143
ATCAACG38250.033.984873
TCAACGC38500.033.7641874
AGTATCG1450.033.25139634
CAACGCA39400.032.9957545
AACGCAG39600.032.742496
ATCTCGT958.785719E-932.68827437
TGCAGTA23000.032.3559315
ACCGATC3200.032.18539427
GCAGTAA23250.032.0093916
GTCGGAT552.9230578E-431.94749859
Produced by FastQC (version 0.11.5)