FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329512
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGTGCTACTACCTGCATATGAGCAGCAATAATAAT84820.6379784462268863No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGTGCTACTACCTGCATATGAGCAG79670.5992424288009436No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61160.46001841277100175No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCACCAGTGATGTT60780.45716022119394184No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG54270.40819488654483754No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG49640.3733700786453977No Hit
CTCCTGCACTGGAACCACCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC49390.3714896894499636No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45940.3455403185529728No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45290.3406513066448441No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC43040.32372780388593714No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC41450.31176852860297616No Hit
GTCGATCACCATCTCCTGCACTGGAACCACCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCA39870.2998844688878325No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG37320.28070449909440454No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35910.27009910403215615No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC35100.2640066430389496No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32730.24618055346623421No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT32550.24482667324552168No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA31680.23828291884541095No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGTGCTACTAC31350.2358008051074379No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30960.2328673979625607No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA30780.2315135177418481No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC30340.22820403275788412No Hit
GTGCTACTACCTGCATATGAGCAGCAATAATAATCAGCCTCGTCGTCAGC29750.22376631425665958No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGC28530.2145900149829411No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27770.20887363182882138No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGCA27400.2060906558195789No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27170.20436069775977952No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCA27060.2035333265137885No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC26650.20044948823327657No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26510.19939647028383348No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26240.19736564995276462No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25560.19225099134118384No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24750.1861585303479773No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC23910.1798404226513187No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23700.17826089572715403No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23180.17434968620065106No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22320.1678811473683577No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC22310.16780593180054035No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22220.16712899169018408No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21690.16314256659586376No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21540.16201433307860327No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC21490.16163825523951644No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21360.1606604528578907No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGTGCTA20670.15547057867849257No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20490.15411669845778No Hit
CCCAAGTGCTACTACCTGCATATGAGCAGCAATAATAATCAGCCTCGTCG20140.15148415358417225No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20120.15133372244853752No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19970.15020548893127705No Hit
GATTATTATTGCTGCTCATATGCAGGTAGTAGCACTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT19720.14832509973584293No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC19640.147723375193304No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19390.1458429859978699No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC19130.14388738123461842No Hit
ATAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG18930.14238306987827112No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT18870.14193177647136693No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18850.14178134533573222No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA18820.1415556986322801No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18760.14110440522537593No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18610.13997617170811547No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18500.13914880046212447No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18390.13832142921613344No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18240.13719319569887298No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC17660.13283069276546583No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT17270.1298972856205886No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16970.12764081858606766No Hit
CCCCTGAGGGCCGCTTACTGCCCTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA16920.12726474074698085No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCG16790.1262869383653551No Hit
ACCTGCATATGAGCAGCAATAATAATCAGCCTCGTCGTCAGCCTGGAGCC16580.12470741144119046No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTATTGCTGCTCATAT16580.12470741144119046No Hit
GCACTGGAACCACCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTAC16480.1239552557630168No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16440.12365439349174734No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG16400.1233535312204779No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16280.12245094440666952No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16220.12199965099976534No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC15940.11989361510087912No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15770.11861495044798392No Hit
TAGTAGCACTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA15680.11793801033762763No Hit
ACTTGGTCCCTCCGCCGAACACCCAAGTGCTACTACCTGCATATGAGCAGCAATAATAATCAGCCTCGTCGTCAG15150.11395158524330731No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC14980.11267292059041212No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14910.11214641161569057No Hit
GGCAAAGCCCCCAAACTCATGATTTTTGAGGGCAGTAAGCGGCCCTCAGG14840.11161990264096902No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14790.11124382480188219No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACC14630.11004037571680436No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14550.10943865117426545No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14370.10808477095355289No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14050.10567787278339721No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13970.1050761482408583No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAC13870.10432399256268465No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13760.10349662131669364No Hit
CCCTGACAATCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTATTGC13670.10281968120633737No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13450.10116493871435535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)