FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3693412
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTTGGCAGTAATAAGTTGCAAGATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC185560.5024080714526297No Hit
GTATAGTAGTTATCCGACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT184340.49910489271167147No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT134220.363403811976568No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCGGTTCATTTGCAGAAAGA108790.2945514878924962No Hit
GTGTAGCATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAG94430.25567144959728294No Hit
GTCTATTACTGTGCGAGAGATTGCAGTCATGGTAGCTGCTACTCCAACTATTACTACGGTATGGACGTCTGGGGC92120.2494170701779276No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC88290.23904725495016532No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG84050.22756735506355644No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC82700.22391219826003705No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC82380.22304579072142508No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC80630.21830762449464072No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT79410.21500444575368252No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG75390.20412020104986933No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG73590.19924665864517688No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC72560.19645790938026952No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC71200.19277567734116854No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA70000.18952664907137357No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT67990.18408452671946698No Hit
GAGTATTGTTACCTGGGTGGCCTGGTATCAGCAGAAACCAGGGGAAGCCCCTAAGCTCCTGATCTATAAGGCGT67640.18313689347411014No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC65170.1764493102854488No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGTTACCTGGGTGGCCTGGTATCAGCAGAAA63430.17173821929424607No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT61530.16659392453373736No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG59360.16071859841252478No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC59270.16047492129229016No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTAGCAT58770.1591211595132089No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT58560.1585525795659948No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATCTTGCAACTTATTACTGCCAACAGTATAGTAGTTAT57070.1545183694643327No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT54990.14888672046335477No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG51770.14016849460607156No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT50110.13567400549952185No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG49970.1352949522013791No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC48780.13207299916716575No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC48390.1310170649794824No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT46950.12711823105572842No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT46750.12657672634409592No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45980.1244919332043108No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG45290.1226237419491787No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG45130.12219053817987272No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA44460.12037649739590384No Hit
GTACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG44160.1195642403284551No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43540.11788557572239436No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG43440.11761482336657811No Hit
CCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGCAAGTGGGGTCCCA42680.11555710546237463No Hit
CAGTATAGTAGTTATCCGACGTGGACGTTCGGCCAAGGGACCAAGGTGGA42250.11439287033236475No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA42210.11428456939003827No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG41280.11176657248094717No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG41270.11173949724536553No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT41060.11117091729815141No Hit
GATCTATAAGGCGTCTAGTTTAGCAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA40990.11098139064908005No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC39910.10805726520626456No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTAGCATCCAGATGACCCAGTCTCCTTCCACC39590.10719085766765256No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT39540.10705548148974445No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA39350.10654105201369356No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA39350.10654105201369356No Hit
ATTGTTACCTGGGTGGCCTGGTATCAGCAGAAACCAGGGGAAGCCCCTAA38290.10367107704204134No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38270.10361692657087809No Hit
ACTATACTGTTGGCAGTAATAAGTTGCAAGATCTTCAGGCTGCAGGCTGC38050.10302127138808236No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG37970.10280466950342934No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA37960.10277759426784773No Hit
GTAGTTATCCGACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA37060.10034082306550149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8300.045.1255151
TCTGGAG37450.040.76915470
GTATCAA60800.040.7479251
TATCAAC68750.035.936022
ATCAACG72050.034.1947143
TCAACGC72700.034.0789644
CGTTGTC28000.033.3517163
CAACGCA74300.033.345555
GCGTTGT28300.033.19833862
AACGCAG76250.032.5837946
GTTGTCT29750.031.9159564
TCGTATG3400.031.53509740
GGCGTTG30800.030.9259261
TTGTCTC30950.030.8861965
AAGAGTG33500.030.17159547
TGGCGTT31550.030.14024760
GCGGTTC33550.029.96848131
CGTATGC3700.029.93051541
CGCAGAG83150.029.8758628
AGAGTAC82700.029.663711
Produced by FastQC (version 0.11.5)