FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3693412
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTTGGCAGTAATAAGTTGCAAGATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC184420.49932149459632447No Hit
GTATAGTAGTTATCCGACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT173380.46943043451421074No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT134970.36543445464518987No Hit
GTCTATTACTGTGCGAGAGATTGCAGTCATGGTAGCTGCTACTCCAACTATTACTACGGTATGGACGTCTGGGGC88380.23929093207039992No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCGGTTCATTTGCAGAAAGA87840.2378288693489922No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT85140.23051855574195348No Hit
GTGTAGCATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAG81720.22125882517303785No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC81700.2212046747018746No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC81120.21963431103814035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG79710.21581670282113125No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC77260.2091832701036332No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC76460.20701725125710319No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC75340.2039848248719612No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC71470.19350670870187242No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA64900.17571827892474493No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT64170.17374178672728632No Hit
GAGTATTGTTACCTGGGTGGCCTGGTATCAGCAGAAACCAGGGGAAGCCCCTAAGCTCCTGATCTATAAGGCGT62610.16951804997655284No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT62110.1681642881974716No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG61250.16583581793745186No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC61080.16537553893256424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57900.15676561401760758No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT57630.1560345826569037No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGTTACCTGGGTGGCCTGGTATCAGCAGAAA56680.15346243527664935No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG56490.15294800580059847No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT56470.15289385532943522No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC54740.14820983957381412No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTAGCAT54600.1478307862756714No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT53520.14490666083285592No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATCTTGCAACTTATTACTGCCAACAGTATAGTAGTTAT52830.1430384695777238No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT49790.13480759796090985No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG48620.13163979539785975No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT47740.12925717466667677No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA47420.12839076712806477No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC45050.1219739362952197No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44520.1205389488093936No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC44490.12045772310264871No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA44240.1197808422131081No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG44040.1192393375014756No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42830.115963233996099No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG42530.11515097692865026No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG42460.1149614502795789No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG40960.11090016494233515No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA40920.11079186400000866No Hit
GTACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG40890.11071063829326379No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA40770.11038573546628429No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40400.10938395174976419No Hit
GATCTATAAGGCGTCTAGTTTAGCAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA40130.10865292038906031No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39700.10748868525905045No Hit
CCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGCAAGTGGGGTCCCA39580.10716378243207095No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG38600.1045104093450717No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG38330.10377937798436786No Hit
CAGTATAGTAGTTATCCGACGTGGACGTTCGGCCAAGGGACCAAGGTGGA38280.10364400180645972No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38210.10345447515738836No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG37840.10245269144086823No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT37320.10104477919062374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8650.042.8946571
GTATCAA60750.038.4554751
TCTGGAG34800.037.27420470
TATCAAC67700.034.2851752
ATCAACG71350.032.654853
TCAACGC71600.032.441914
CAACGCA73450.031.67155
AACGCAG74900.031.0583696
GCGTTGT27150.030.75698762
CGTTGTC28000.029.99416463
GTTGTCT29300.029.0760864
TGTGCGA26950.029.02691810
TGGCGTT29100.028.61083260
TTGTCTC30300.028.56248565
TGCGAGA28300.028.00557712
AGAGTAC83550.027.96531111
TTGGCGT29250.027.94922359
CGCAGAG83400.027.9340868
CTGTGCG28700.027.8551049
GGCGTTG29800.027.75493261
Produced by FastQC (version 0.11.5)