FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501335
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT400.00934826748.6997470
GTATCAA14300.038.2018281
GTGGTAT2500.035.7319261
TATCAAC17000.032.134482
TCAACGC17400.031.5932144
ATCAACG17450.031.3057983
CAACGCA17600.031.2342035
AACGCAG18000.030.5401086
GGTATCA7700.030.3417931
AGTACGG15000.029.0894513
CTTATAC1202.7976057E-928.6313511
GTAGATC605.550593E-428.6313513
AGAGTAC19300.028.30498311
ACGCAGA19500.028.1908687
GAGTACG15400.028.11077912
TAACGGA500.00719575928.00849757
CCCGTAA600.007210818627.99540969
CGCAGAG20000.027.6578878
CAGAGTA19950.027.38276310
TCGTATG658.476472E-426.63080640
Produced by FastQC (version 0.11.5)