FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501335
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10490.2092413256604865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAA400.00856171949.79475870
TTGCGTA305.083665E-448.05801467
CGCTAGC306.491909E-445.73254819
GTATCAA14100.038.944631
GGTATCA6350.037.292551
TATCAAC15350.035.7589652
TCAACGC16900.032.8688854
ATCAACG16900.032.6659933
AACGCAG17450.032.4224056
CAACGCA17550.031.651525
ACGCAGA18200.031.0863157
CACGACT450.00436568631.02384850
TCTAGAC450.00476087130.4792443
CGCAGAG18700.030.255138
AGAGTAC18750.029.808711
CAGAGTA19200.029.11005810
GCAGAGT19750.028.6466319
GTGGTAT2100.027.782821
CGACCTA500.007709129727.61586436
AGTACGG14250.025.02506313
Produced by FastQC (version 0.11.5)