FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776089
Sequences flagged as poor quality0
Sequence length20-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.18928241477459415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGA200.007517215351.4707535
ACGCGTA1850.040.5528927
GTATCAA37050.040.5352821
AGACGCG1950.040.198725
CCGTTAA350.001436306438.92017716
GACGCGT1950.038.4608526
GCGTAGT1900.037.71523729
CGCGTAG1900.037.6957728
CGAGACG2200.037.16544323
TATCAAC40850.036.683562
TCGTATG1800.036.5571840
CCCACGA2300.035.53581219
ATCAACG42800.034.8530853
TCAACGC42900.034.771844
TATGCCG2050.033.9485743
CAACGCA44200.033.6742555
GCCGTCT1900.033.10315346
AACGCAG45750.032.6078266
AATCTCG1900.032.55222336
GTGGTAT5450.032.4929921
Produced by FastQC (version 0.11.5)