FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899709
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41100.040.9672431
GGTATCA16750.038.898771
TCGTATG753.9313818E-836.89219740
GTGGTAT4900.036.3918651
TATCAAC47150.035.8560332
TCAACGC49250.034.3986934
ATCAACG49100.034.3621753
CAACGCA49850.034.053465
AACGCAG51800.032.705326
ACGCAGA54650.031.0624877
CGCAGAG54850.031.011758
AGAGTAC55050.030.2121411
CAGAGTA55900.029.69304810
TGGTATC5850.029.309692
GCAGAGT57900.029.200459
AATAGCG1352.2919266E-1028.81799162
TACGCAA950.00879390426.88036770
AGAGTCG1306.722985E-926.3787211
AGTACGG38550.026.1529113
GAGTACG38600.026.11903212
Produced by FastQC (version 0.11.5)