FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437384
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC900.049.4469531
CTCGTAT751.6370905E-1146.11791639
GTATCAA17450.041.981411
TCGTATG955.456968E-1240.09145440
GGTATCA6600.038.9005741
TATACAC1150.038.693193
CGTATGC1009.094947E-1238.14864741
GTGGTAT2150.036.620821
TATCAAC20000.036.4534532
ATCAACG21700.033.282183
CAACGCA22150.032.7605485
AGACTAG1056.9303496E-1032.61730625
TCAACGC22250.032.4594764
AACGCAG22500.032.403076
ATAGGAC652.446844E-531.5956253
TCTCGTA1101.0459189E-931.38601938
TTATACA1450.030.6877042
GTATACA450.004801545830.4254134
ACGCAGA24250.030.0647037
TGGTATC2650.029.7078362
Produced by FastQC (version 0.11.5)