FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437384
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10100.23091836921332287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19100.043.339281
GGTATCA7100.040.4688221
GTGGTAT2200.038.8702161
TATCAAC21850.038.0107732
TCAACGC24550.033.9579284
ATCAACG24600.033.888913
AACGCAG25050.033.689316
CAACGCA24950.033.6873935
ACGCAGA26900.031.6264117
TATTACC553.4374138E-431.0712812
CGCAGAG27550.030.756228
TGGTATC2800.030.5164362
TAAACGT450.004828011630.39123520
AGTGTAA450.004844244630.3703910
AGAGTAC27550.030.13613511
CAGAGTA27750.030.0420610
GCAGAGT28600.029.6270589
GAGTACG19550.028.83633212
GTATAGG605.6951126E-428.5048261
AGTACGG19550.028.48680113
Produced by FastQC (version 0.11.5)