FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109706
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2390.2178549942573788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT200.00793118150.7482920
GTGGTAT1000.040.5801281
CGGGGAG451.1200961E-437.5741964
GGTATCA3500.034.7829671
GTATCAA7300.034.2800141
TATCAAC7650.032.711652
ACGGGAT450.005066592730.07306116
CACACTG450.005077909730.0593555
ATTCTGA604.4957118E-429.65387752
ATCAACG8450.029.6146893
AACGCAG8400.029.3883866
CAACGCA8550.028.8727995
TCAACGC8550.028.8727994
ACGCAGA8950.027.5823977
CGCAGAG9000.027.429168
TGGTATC1504.3655746E-1127.053422
TCTAGAC500.00849476427.0534173
ACGGGGC500.00849476427.0534175
AGAGTAC9050.026.90395211
CAGAGTA9300.026.54434810
Produced by FastQC (version 0.11.5)