FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998297
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTTATTTGCAGATACA24680.24722101739261962No Hit
GTATAGTAACTGGCCTGTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23900.2394077113323991No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22800.22838894637567778No Hit
GTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20090.20124271634593713No Hit
GTGTATTACTGTGCGAGGGACTATGGTTCGGGGAGTTATCAAAGCATAAG18310.18341235123415175No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17960.17990638056610408No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17030.17059051564814878No Hit
GTTACTATACTGCTGACAGTAATAAACTGCAACATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT16520.16548181553185073No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15870.1589707271483336No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15360.15386202703203555No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATG14530.14554786801923675No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14360.14384496798047075No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13430.13452910306251548No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13380.1340282501099372No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13380.1340282501099372No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13240.13262586184271816No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13150.1317243265280773No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12940.1296207441272487No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATATCAGGTGCCCGGGT12770.1279178440884827No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12720.12741699113590443No Hit
CTCCTGCAGGGCCAGTCGGAGTGTTAGCAGCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGACT12280.12300948515321591No Hit
AGTGTACACAGGCCAGTTACTATACTGCTGACAGTAATAAACTGCAACAT12090.12110624393341862No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11980.12000436743774649No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11000.11018764956721296No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10970.10988713779566603No Hit
CCTGATATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGT10310.10327587882163325No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10080.10097195523977333No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10070.1008717846492577No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10050.10067144346822639No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10010.1002707611061638No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACACAGGCCAGTTACTATACTG10010.1002707611061638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1000.055.34961740
GTCGTAT408.1368853E-751.8667239
CTCGTAT901.200533E-1038.41979239
CGGGTTT700.001603430138.05559570
AATCGAG700.001603430138.05559570
GTATCAA17100.037.172621
CGTATGC1500.036.93327341
CTTATAC2150.036.7567371
TATACAC2500.035.732153
ACGAGAC2300.034.36472322
TATCAAC19800.031.754972
TCCGTTC553.3375155E-431.23283413
GTCGTGA450.00432676831.08371556
GACCCTA2550.031.00961526
TCAACGC20550.030.763224
ATCAACG20850.030.4853733
CCCACGA2600.030.39194919
AACGCAG21150.030.2199446
CAACGCA21200.030.145655
TGTTAGA4000.029.9687870
Produced by FastQC (version 0.11.5)