FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998297
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTTATTTGCAGATACA24350.24391538790560324No Hit
GTATAGTAACTGGCCTGTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24150.24191197609529028No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23060.23099338172908462No Hit
GTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG21130.21166045775956455No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18750.18781985721684027No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17740.1777026275747598No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16790.16818642147577323No Hit
GTTACTATACTGCTGACAGTAATAAACTGCAACATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT16690.16718471557061676No Hit
GTGTATTACTGTGCGAGGGACTATGGTTCGGGGAGTTATCAAAGCATAAG16590.16618300966546026No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15520.15546475648028593No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATG14750.14775162101058104No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14210.14234240912273602No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13360.13382790892890592No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATATCAGGTGCCCGGGT12960.12982108530828002No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12690.12711647936435752No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12680.12701630877384185No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12560.12581426168765408No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12500.12521323814456017No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12230.1225086322006377No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12170.1219076086575438No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11990.12010453802826214No Hit
AGTGTACACAGGCCAGTTACTATACTGCTGACAGTAATAAACTGCAACAT11550.11569703204557362No Hit
CTCCTGCAGGGCCAGTCGGAGTGTTAGCAGCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGACT11240.11259174373958852No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11220.11239140255855723No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10460.10477843767936795No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACACAGGCCAGTTACTATACTG10310.10327587882163325No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10120.10137263760183592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17650.038.3807141
CTTACGA400.002649406234.3574832
TATCAAC21000.032.3941962
TCAACGC21200.031.745374
ATCAACG21450.031.3816663
CAACGCA21800.031.0291565
AACGCAG22000.030.7470726
CGGTCGT900.00494084430.24889870
AGAGTAC23300.028.88275311
TGTTAGA4500.028.73645270
GTATAGA605.526976E-428.6556511
GTGGTAT4300.027.1895481
GCAGAGT24950.027.1116479
CGCAGAG24950.026.9740248
TGTGCGA7950.026.7784110
CAGAGTA25050.026.72926910
TGTGATT5350.026.71514570
ATAATAC658.888839E-426.4182423
ACGCAGA25850.026.167727
TCTGGAG13350.026.00045670
Produced by FastQC (version 0.11.5)