FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645449
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78200.2956019942172387No Hit
GTAATATATAGCCGAGTCCTCGGGTCTCAGGCTGTTCATTTGCAAATACA77410.2926157336618472No Hit
ATATATTACTGTGCGAGAGATCGGGGATTCAGTTTATGGAGTGGAGCCCT68590.25927545758772896No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC52870.19985265261208968No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50370.19040246098110378No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47560.17978044558787562No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT44550.1684024148641686No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44400.16783540336630945No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT44060.16655017730449537No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43650.1650003458770137No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43010.1625810968194813No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG42080.15906562553275455No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAG41430.1566085757086982No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41360.15634397034303063No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT40900.15460513508292922No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39740.15022024616615176No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC39540.1494642308356729No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39200.1481790047738588No Hit
GTCTGCATCTGTGGGCGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG38620.14598656031547008No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG37510.14179067523131236No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG36510.13801059857891798No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG34800.13154666750332364No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGCGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAA34670.13105525753851235No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33890.12810679774964476No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG33340.12602775559082788No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA31280.1182407976868955No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29890.11298649114006734No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT29750.11245728040873214No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA29480.11143665971258565No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29000.10962222291943635No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA28950.10943321908681665No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT28590.10807239149195469No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC28340.10712737232885608No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC27870.10535073630223073No Hit
GTATCAGACGTTTGACAGTAATAAGTTGCAATATCTTCAGGCAGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTC27530.10406551024041664No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27450.10376310410822512No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC27270.10308269031079412No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27250.10300708877774624No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATCCAATAGGCAC27090.10240227651336314No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG26600.1005500389536899No Hit
AGTCAGGACATTAGGAACTCTTTAAATTGGTATCAACAGAAACCAGGGAA26500.10017203128845048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6400.042.4803351
TCGTATG801.5988917E-938.9200840
ATCAACG66500.036.7943043
TCAACGC66850.036.3964354
CAACGCA67800.035.9372145
AACGCAG68850.035.3891536
ACCCTGG33200.032.4517570
CGCAGAG77300.031.467768
AGAGTAC76900.031.31766711
CAGAGTA78400.030.71905510
GCAGAGT79800.030.3956999
TCTGGAG33850.030.10295170
ACGCAGA82300.029.5147427
GTATCAA85700.028.7121721
GAGTACT42350.026.8900912
AGTACTT42350.026.80885113
TATCAAC92500.026.5637342
ACCGATC3450.025.95073927
CTCGTAT1208.350435E-825.93094839
TTCGGGG23900.025.7984670
Produced by FastQC (version 0.11.5)