FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645449
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76600.28955387157340773No Hit
GTAATATATAGCCGAGTCCTCGGGTCTCAGGCTGTTCATTTGCAAATACA73150.27651260712264725No Hit
ATATATTACTGTGCGAGAGATCGGGGATTCAGTTTATGGAGTGGAGCCCT65910.2491448521593121No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT51580.19497635373050096No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47850.18087666781707No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC47770.18057426168487845No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT47270.17868422335868128No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45230.17097286698779676No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG44270.1673439934014982No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43630.1649247443439658No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43420.16413092824696301No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAG39280.14848141090605035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38330.14489033808627572No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT37960.14349170972488978No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37780.1428112959274588No Hit
GTCTGCATCTGTGGGCGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG37760.14273569439441094No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36780.13903121927506445No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC34910.131962475935087No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34570.13067724987327292No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGCGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAA33760.1276153877848335No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG32570.12311709656848421No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32490.12281469043629266No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA32180.12164286667405043No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA31360.11854320381908705No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31040.11733357929032086No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG30250.11434731873492932No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30220.11423391643535748No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30030.11351570187140254No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29590.11185246814434903No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29510.11155006201215749No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT27620.10440571713913215No Hit
GTATCAGACGTTTGACAGTAATAAGTTGCAATATCTTCAGGCAGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTC27560.1041789125399885No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT27170.10270468264555468No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATCCAATAGGCAC26870.10157065964983639No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC26620.1006256404867378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6950.036.606961
TCAACGC65100.035.4990734
ATCAACG65600.035.284183
CAACGCA65950.034.78125
TATATCG400.002657337334.3386275
ACCCTGG28400.034.14257470
AACGCAG67350.034.109196
TCTGGAG35350.030.8350670
CGCAGAG75450.030.3563468
AGAGTAC75150.030.3404511
CAGAGTA75900.030.08588210
GTATCAA79750.029.7895641
GCAGAGT78350.029.3204089
ACGCAGA81200.028.122157
CCGTTAG500.00786840427.504691
TATCAAC87500.027.1346262
TGTGCGA22900.025.19165610
AGTACGG52950.025.09735113
GAGTACG53850.024.67789812
CATCAAT9000.024.51986170
Produced by FastQC (version 0.11.5)