FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519053
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT801.6225385E-938.8478339
GTATCAA14800.037.7963641
CGTATGC952.1282176E-1036.4163341
TAGCGCT400.002367301835.1519653
TCGTATG1003.7289283E-1034.5583740
GGTATCA6550.032.8070451
TATCAAC17400.032.3446582
CAACGCA17700.031.9922315
ATCAACG17800.031.8094353
TGGTATC3450.031.637252
TCAACGC17900.031.4442044
CCGGTCG850.004595790530.69491470
AACGCAG18600.030.4442216
TATACTG902.199049E-730.3219535
GTGGTAT3600.030.319031
TCTCGTA1052.3359462E-829.55798538
AGAGTAC19600.028.5401111
CGCAGAG20200.028.0327958
CAGAGTA20350.027.65853910
GGTACGG500.00817329227.287133
Produced by FastQC (version 0.11.5)