FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519053
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9920.19111728474741502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGC252.6834736E-454.7014229
AAATCGG350.001345502739.4382139
GTATCAA15150.035.378281
TAGCGTC400.002751854734.0895847
GGTATCA7400.033.6775781
TATCAAC16950.031.1974122
ATCAACG17800.029.8762653
TCAACGC18000.029.544314
CAACGCA18200.029.2196485
AACGCAG18450.029.0084826
AGAGTAC19500.027.79612511
GTGGTAT3400.027.1103151
CGCAGAG19900.026.89488
CAGAGTA20300.026.8686410
TGGTATC3500.026.3179632
ACGCAGA20500.025.9413457
GCAGAGT21000.025.4860259
GTGTATA951.1385226E-525.1549911
GTCTTAG1101.2612109E-624.8283021
CGATTAA1400.001683889223.66800370
Produced by FastQC (version 0.11.5)