FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205194
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54900.45552832158142176No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT39150.3248439670293745No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36680.3043493412678789No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34580.2869247606609392No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG34010.28219523163905563No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30860.2560583607286462No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC29880.247926889778741No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29860.24776094139200827No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29040.24095705753596516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28350.235231838193685No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC27750.23025338659170225No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGACGTTCGGCCAAGGGA27220.22585575434328417No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26340.21855402532704277No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGGCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT25670.21299475437149537No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25220.20926091567000832No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG22590.18743870281465058No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC22160.18387081249989629No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21980.18237727701930145No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21610.17930723186474543No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21590.17914128347801267No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20900.17341606413573252No Hit
GTCTCTGATGGCTCCCTCAGTAGTGGTGATTTCTACTGGAGTTGGATCCG20350.16885248350058166No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20310.16852058672711612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20100.16677812866642217No Hit
ACTTAGGCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATC20090.16669515447305577No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGGCTGGTA19580.16246347061137045No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19540.16213157383790494No Hit
GCGTAGCAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19530.16204859964453855No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT18760.15565958675532737No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18450.15308738676096958No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17570.14578565774472824No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17380.144209148070767No Hit
TCTATGATGCATCCAACAGGGCCAGTGGCATCTCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACT17210.1427985867835386No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA16980.14089018033611186No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16630.1379860835682886No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16450.13649254808769376No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16330.13549685776729722No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGGCCAACATCAGGGAGA16220.13458414164026702No Hit
CTGCTACTCTGGCTCCCAGTTTCAGATACCACCGGAGAAATGGTGTTGAC16190.1343352190601679No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16180.13425224486680154No Hit
GTCAGAGTGTTGGCAGCTACTTAGGCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATG15910.1320119416459093No Hit
GTGTATTACTGTGCCAGAGGTTTCTTGGAGGGTGCGGTGACTCCGAAACTGGACGTGGTTCTGGGGTTAAAATA15900.13192896745254293No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15870.13168004487244378No Hit
CAACAGGGCCAGTGGCATCTCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG15780.13093327713214636No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15750.13068435455204722No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15700.1302694835852153No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15460.1282781029444222No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15450.12819512875105585No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15330.12719943843065928No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15190.12603779972353No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14540.12064447715471532No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14430.11973176102768517No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14340.11898499328738775No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14290.11857012232055586No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14100.11699361264659465No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCC13400.1111854191109481No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13300.11035567717728433No Hit
CACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGACGTT13290.11027270298391795No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13270.11010675459718518No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13270.11010675459718518No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13180.10935998685688776No Hit
GTGGCATCTCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACT13150.10911106427678864No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATGCATCCAACAGGGCCAGTGGCATCTCATCCAGGTT12940.10736860621609466No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTTTCAG12890.10695373524926277No Hit
CTCCCAGGCTCGTCATCTATGATGCATCCAACAGGGCCAGTGGCATCTCA12880.1068707610558964No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12520.10388369009470674No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGATGAGATGCCACTGGCCCTGTT12470.10346881912787484No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGGCTGGTACCAACAGAAACCTGGCCAGG12430.10313692235440933No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12400.10288799977431021No Hit
CACCTGCACTGTCTCTGATGGCTCCCTCAGTAGTGGTGATTTCTACTGGA12210.101311490100349No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12080.10023282558658607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAT150.00225973269.7265150
TATCGTA306.258995E-446.07813338
GGTATCA9750.042.2054331
CGTATGC1650.041.9910441
GTATCAA25100.040.849731
TCGTATG1550.040.19306640
CTTATAC2700.036.8320541
TATCAAC28750.035.902142
CTCGTAT1550.035.70623839
TATACAC2800.035.5166243
CACGAGA2750.034.924121
ACGAGAC2850.034.9051122
ATCGTAT400.00256326434.59041639
ATATCGT400.002585965234.52828237
CGGCTAT400.002652863334.3489228
TCTCCGA2850.033.691711
ATCAACG30550.033.6745453
ATCTCGT1650.033.48196837
TCAACGC30850.033.2359244
ATCTCCG2900.033.1108110
Produced by FastQC (version 0.11.5)