FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205194
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55580.4611705667303356No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT38270.31754223801313314No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34440.28576312195380993No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32640.27082776714786166No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC30620.25406698008785306No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30320.2515777542868617No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29640.2459355091379479No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29100.24145490269616346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28120.23332343174625828No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC27420.22751523821061176No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG26980.22386437370249104No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26040.2160647995260514No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGACGTTCGGCCAAGGGA25870.21465423823882296No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGATGAGATGCCACTGGCCCTGTT25690.21316070275822813No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23500.19498935441099108No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23040.19117254151613766No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG22960.1905087479692066No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22710.18843439313504715No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGGCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT22110.18345594153306438No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21760.1805518447652411No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20990.17416283187602993No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20540.17042899317454285No Hit
GTCTCTGATGGCTCCCTCAGTAGTGGTGATTTCTACTGGAGTTGGATCCG20350.16885248350058166No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19380.16080398674404286No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19030.1578998899762196No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18500.1535022577278015No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17940.14885570289928426No Hit
GCGTAGCAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17810.14777703838552134No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17780.1475281158054222No Hit
ACTTAGGCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATC17660.14653242548502565No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC17610.14611755451819375No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17370.14412617387740065No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGGCTGGTA16820.1395625932422498No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA16310.13533090938056444No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16170.13416927067343515No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16130.13383737389996964No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16070.13333952873977134No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15890.1318459932591765No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCGCTGAACCTGGATGAGATGCCA15830.13134814809897824No Hit
GTGTATTACTGTGCCAGAGGTTTCTTGGAGGGTGCGGTGACTCCGAAACTGGACGTGGTTCTGGGGTTAAAATA15830.13134814809897824No Hit
TCTATGATGCATCCAACAGGGCCAGTGGCATCTCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACT15720.1304354319719481No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15380.1276143093974912No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15060.12495913520976706No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGGCCAACATCAGGGAGA14870.12338262553580587No Hit
CAACAGGGCCAGTGGCATCTCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG14770.12255288360214206No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14640.12147421908837915No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14590.12105934812154724No Hit
CTGCTACTCTGGCTCCCAGTTTCAGATACCACCGGAGAAATGGTGTTGAC14530.12056150296134896No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14530.12056150296134896No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14440.11981473522105154No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14200.11782335458025844No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13820.11467033523233604No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13760.11417249007213777No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13420.11135136749768086No Hit
GTCAGAGTGTTGGCAGCTACTTAGGCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATG13400.1111854191109481No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13360.1108535223374826No Hit
GTGGCATCTCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACT13280.11018972879055156No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13110.10877916750332312No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13040.10819834814975846No Hit
CACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGACGTT12820.10637291589569813No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12720.10554317396203432No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12630.10479640622173693No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG12480.10355179332124122No Hit
CACCTGCACTGTCTCTGATGGCTCCCTCAGTAGTGGTGATTTCTACTGGA12350.1024731288074783No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12180.10106256752024985No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12130.10064769655341796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.047.5716931
GTATCAA24050.042.7749631
TATCAAC28100.036.338652
GTTATTA951.3789513E-436.30268570
ATCAACG30250.034.190553
TCAACGC30700.033.464894
CAACGCA31250.032.8772745
AACGCAG31600.032.6215026
CGTGAAA450.004326800431.08418755
CGCAGAG34000.030.1173558
AGAGTAC34600.029.89202711
AATTGAG7950.029.49878370
AGCGTAC853.705598E-629.06952363
GCAGAGT35500.029.0377319
CGCGCTA756.38394E-527.45810528
ACGCAGA38150.027.0206957
CAGAGTA38350.026.79047610
CAGTTCG23650.026.54010270
TATAGAC659.0372463E-426.3439673
TGTTAGA8350.025.6075270
Produced by FastQC (version 0.11.5)