FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636442
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10070.1582233730646312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30150.043.600291
GTGGTAT4200.039.1234741
TATCAAC34050.038.3751452
GGTATCA11150.037.76381
ATCAACG36850.035.248663
TCAACGC37150.034.9557764
CAACGCA37400.034.722115
AACGCAG38550.034.0408976
TGGTATC5000.031.469632
ACGCAGA42000.031.3260467
TCTAGAC553.437085E-431.0744783
CGCAGAG42950.030.474028
AGAGTAC43250.029.9465811
CAGAGTA43400.029.7643410
GCAGAGT45700.028.5654689
AGTACGG29850.028.04890413
GAGTACG30350.027.58681312
GTACGGG32300.025.18074614
TACGGGT4100.025.009215
TACGGGG12850.025.00271215
Produced by FastQC (version 0.11.5)