FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721888
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26750.37055609734474043No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21150.29298173677911254No Hit
CACTATCAACAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAAC20560.2848087237909482No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19510.27026353118489294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19210.2661077618688772No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG18330.2539175052085642No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17600.24380513320625916No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTC15990.22150250454364115No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15530.21513032492575024No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15490.2145762223502815No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15280.21166718382907043No Hit
GTTGATAGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTCG14610.2023859656899685No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14410.19961545281262466No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13730.19019570902965557No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTCAGACGTGTCTACTGA13590.18825635001551488No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA12600.17454231127266281No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12240.16955538809344384No Hit
GGATATTAGCAGCTGGTTAGCCTGGTATCAGCAGCGACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC12180.1687242342302407No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12170.1685857085863735No Hit
CTATCAACAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAG11930.16526109313356088No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11770.1630446828316858No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11740.16262910590008423No Hit
GGTATCAGCAGCGACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCAT11730.16249058025621702No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCGAGGTT11650.1613823751052795No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGGGAGGGAAA11440.15847333658406845No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA11400.15791923400859967No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11370.1575036570769981No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT11290.15639545192606055No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTTCTGGAGTTGGATCCG11190.15501019548738862No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10940.1515470543907088No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10310.14281993882707567No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10070.13949532337426304No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT9990.1383871182233255No Hit
GTGTATTACTGTGCGAGAGGAGACGCGCGCATCCCGGGCGGTGACTACGA9990.1383871182233255No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9580.13270756682477058No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9530.13201493860543465No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9220.12772064364555166No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9220.12772064364555166No Hit
ATTGTCAACAGGCTGACAGTTTCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG8960.12411897690500465No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8870.12287224611019992No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8810.12204109224699677No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8740.12107141273992642No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8580.11885500243805133No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8520.11802384857484817No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8520.11802384857484817No Hit
GGCCAAAAGTGGGAGGGAAACTGTCAGCCTGTTGACAATAGTAAGTTGCA8430.11677711778004345No Hit
GCTCCTGGGACTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC8260.11442218183430115No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGTTGATAGTGAGAGTGAAATCTGTCCCAGATCCA8190.11345250232723081No Hit
GATTTCACTCTCACTATCAACAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTGACAGTTTC8160.11303692539562923No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCC8130.11262134846402765No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTCGCTGCTGATACCAGGC8050.11151314331309013No Hit
ATCAACAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTGACAGTTTCCCTCCCACTTTTGGC7960.11026641251828538No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7940.10998936123055099No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7910.10957378429894943No Hit
GTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT7870.10901968172348066No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7800.10805000221641031No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7720.10694179706547276No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7520.1041712841881289No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7330.10153929695465225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)