FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721888
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25500.3532403918613414No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG20170.27940622368012763No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19550.2708176337603617No Hit
CACTATCAACAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAAC18430.25530276164723614No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17480.24214282547985283No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16990.2353550689303604No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16880.23383128684782128No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16490.22842878673700076No Hit
GTTGATAGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTCG15890.2201172481049692No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15050.20848109402012502No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14710.20377122212864046No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTCAGACGTGTCTACTGA13820.1914424398244603No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTC13640.18894897823485082No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13610.18853340130324928No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13280.1839620550556319No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13130.181884170397624No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA12830.17772840108160823No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12560.17398820869719403No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCGAGGTT12360.1712176958198502No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12190.1688627598741079No Hit
GGATATTAGCAGCTGGTTAGCCTGGTATCAGCAGCGACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC11910.16498404184582652No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTTCTGGAGTTGGATCCG11480.1590274391595372No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11460.15875038787180284No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10960.1518241056784432No Hit
GGTATCAGCAGCGACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCAT10850.15030032359590406No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGGGAGGGAAA10810.1497462210204353No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10430.144482246553482No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA10360.14351256704641163No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10260.1421273106077397No Hit
GTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT10120.140187951593599No Hit
GTGTATTACTGTGCGAGAGGAGACGCGCGCATCCCGGGCGGTGACTACGA10020.1388026951549271No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT9860.136586284853052No Hit
CTATCAACAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAG9840.1363092335653176No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGG9830.13617070792145042No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9750.13506250277051288No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9750.13506250277051288No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT9530.13201493860543465No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9480.13132231038609868No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9210.12758211800168445No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9110.12619686156301255No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9000.12467307948047343No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8730.12093288709605922No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8720.12079436145219202No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8630.1195476306573873No Hit
ATTGTCAACAGGCTGACAGTTTCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG8570.11871647679418412No Hit
GGCCAAAAGTGGGAGGGAAACTGTCAGCCTGTTGACAATAGTAAGTTGCA8560.11857795115031695No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8480.11746974599937941No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGTTGATAGTGAGAGTGAAATCTGTCCCAGATCCA8150.11289839975176204No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8050.11151314331309013No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8000.11082051509375415No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCC7830.10846557914801187No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7820.10832705350414468No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7680.106387694490004No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7660.10611064320226961No Hit
ATCAACAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTGACAGTTTCCCTCCCACTTTTGGC7520.1041712841881289No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7450.10320160468105856No Hit
GATTTCACTCTCACTATCAACAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTGACAGTTTC7440.10306307903719136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12750.043.3426321
CTGGAAT6200.036.29694770
GTGGTAT2000.036.040341
ATCAACG15150.035.9859123
TCAACGC15150.035.9809274
AACGCAG15350.035.2887736
CAACGCA15700.034.720455
AGAGTAC16950.032.15994311
CGCAGAG17350.031.2209038
CAGAGTA17500.031.14920610
GCAGAGT18000.030.2839499
TTCGGGG3850.030.11175570
ACGCAGA18700.029.3336627
TCGCTAT953.3678043E-728.884524
CTCGCTA953.3739525E-728.87849823
ACTGGAT10650.028.17415670
GTATAAG1155.9191734E-826.8623641
GTGTATA809.894815E-525.74311
AGTACTT4750.025.26168413
GAGTACG17050.024.9336112
Produced by FastQC (version 0.11.5)