FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530742
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTG11690.22025767698806578No Hit
TCTCTGGGCTGCAGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT10800.20348870072464587No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC10250.19312585022477965No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10130.19086486466117247No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTGCCTGCATATGAGCTG9500.1789946904522348No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8850.16674768531602926No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCACCATCTCCTG7760.14621039977993072No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC7390.1392390276254753No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC7320.13792011938003776No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7250.13660121113460022No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6940.13076033176194837No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6930.13057191629831444No Hit
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAACA6620.12473103692566256No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGC6560.12360054414385897No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG6560.12360054414385897No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG6440.12133955858025179No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6430.12115114311661787No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6380.1202090657984482No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5800.10928096890768019No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC5480.10325167407139438No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5400.10174435036232293No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5350.10080227304415326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAT200.0075033651.49255423
GGTATCA4050.039.845371
GTATCAA10400.039.6171261
TATCAAC13250.031.3548372
GCCGCTT4950.030.89674270
TCAACGC13800.030.353994
GTGGTAT2050.030.1476651
ATCAACG13700.030.0743143
CAACGCA14400.028.8508075
AACGCAG14200.028.7735636
AATGGTA950.007203494727.99796370
TGGTATC2000.027.4678752
CGCAGAG15150.026.9641978
GGGAACG2256.483788E-826.59806370
CAGAGTA15750.026.59086410
AGAGTAC15900.026.12410511
TATAGGG3300.026.0112445
ATTGTCT3100.025.11214464
GGTCATT3300.025.0581734
AGCGTGT2650.024.9728150
Produced by FastQC (version 0.11.5)