FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530742
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC10580.1993435605246994No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTG10200.19218377290660998No Hit
TCTCTGGGCTGCAGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT10090.19011120280663676No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9350.1761684584977258No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8870.1671245162432971No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCACCATCTCCTG8740.16467511521605602No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7660.14432624514359144No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTGCCTGCATATGAGCTG7600.14319575236178783No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7330.1381085348436717No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7100.13377497918009126No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6920.1303835008346805No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6720.12661519156200188No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGC6650.12529628331656434No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC6650.12529628331656434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG6500.12247005136205538No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6480.12209322043478753No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG5820.10965779983494806No Hit
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAACA5740.1081504761258766No Hit
CTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCT5650.10645473695317122No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5540.10438216685319797No Hit
CTCCTGCACTGGAACCACCAGTGACGTTGGTGATTATGAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC5410.10193276582595685No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5390.101555934898689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11300.041.9645771
GTGGTAT1850.040.8632741
TATCAAC12150.037.5934142
ATCAACG13000.036.16143
AACGCAG13400.036.1062476
CAACGCA13300.035.8617255
TCAACGC13250.035.479114
TGGTATC2050.035.180492
GGTATCA4500.035.1259041
CGCAGAG14700.033.1466038
AGAGTAC14700.032.6797511
GCAGAGT15300.032.071019
CAGAGTA15000.032.02615410
GTCTTAC652.3834511E-531.7190461
TAGATTG450.00474445630.50115
GTACTAT605.546594E-428.635251
CGAACTA854.5787947E-628.2850229
CAAATAG500.007495687327.77548245
CCTATAT1750.027.450993
CTAACCC500.00793816927.4509893
Produced by FastQC (version 0.11.5)