FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678622
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAA200.007213223752.0102341
TATTCGG200.007497528651.5042411
GTATCAA19950.039.745941
GGTATCA8300.037.2210041
TCAACGC23800.033.1746674
TGGTATC3650.032.9178052
ATCAACG24250.032.842183
AACGCAG24100.032.764126
CAACGCA24350.032.4253435
GTGGTAT3850.031.205491
TATCAAC25850.031.074992
ACGCAGA25500.030.972157
AGAGTAC26550.029.48641811
CGCAGAG27100.029.2702478
CAGAGTA27200.029.03425210
GCAGAGT28450.027.7585839
TACTCGA500.00792297927.4628542
GAGTACG22550.023.90588412
AGTACGG22850.023.59028213
TTATACA1503.0389856E-822.8857122
Produced by FastQC (version 0.11.5)