FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114300
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41260.37027730413712645No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39050.3504442250740375No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37720.3385084806605044No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34710.31149600646145564No Hit
ATTTTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCAT34450.30916270304226867No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGTTGGAGTTGTATAAAATAGT33660.30207305034550835No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCGGGGGTCCCT33420.29991923180472047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32800.2943552005743516No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31160.27963744054563405No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28550.2562146639145652No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28360.2545095575697748No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27960.25091986000179484No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27040.2426635555954411No Hit
ATTTTATGGCAGCGGGTCTGGAACAGATTTCACTCTCACCATCAGCAGCC26910.24149690388584763No Hit
ATTTTATACAACTCCAACAATAGGAACTACTTAGCTTGGTACCAGCAGAA26870.24113793412904963No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26610.2388046307098627No Hit
CTATAAAATTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTT26430.23718926680427174No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25060.22489455263394062No Hit
GTTTATTACTGTCAGCAATTTTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGAA24630.22103562774836222No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24000.21538185407879387No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23860.2141254599300009No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22630.2030871399084627No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA22120.19851027550928835No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21680.19456160818451046No Hit
GTGCAGCCACAGTTCGTTTAATTTCCACCTTGGTCCCTTGGCCGAACGTC21310.19124113793412906No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21120.1895360315893386No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20840.18702324329175268No Hit
GTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA20050.1799335905949924No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATTTTATAGTGCT19350.17365161985102756No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19230.1725747105806336No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19070.17113883155344162No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18970.17024140716144665No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17850.16019025397110295No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17660.1584851476263125No Hit
GCCATAAAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGTAAA17320.15543390469352958No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17200.1543569954231356No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17180.1541775105447366No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCACCCAGACTATTTTATA17010.15265188907834515No Hit
GGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATTTTATAGTGCTCCGT16870.15139549492955218No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16840.1511262676119537No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16830.15103652517275418No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16500.14807502467917077No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16360.1468186305303778No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGTTGGAGTTG16290.14619043345598134No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16260.14592120613838286No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16190.14529300906398634No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATTTTATAGTGCTCCGTGGACGTT16170.14511352418558734No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA16100.1444853271111909No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15940.14304944808399891No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15750.14134434173920848No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15750.14134434173920848No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15670.14062640222561248No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATTTTATGGCAGCGGGTCTGGAACAGATTT15570.1397289778336175No Hit
GGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC15220.1365879924616351No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15200.1364085075832361No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15010.13470340123844565No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15010.13470340123844565No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14860.13335726465045322No Hit
GTATAAAATAGTCTGGGTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA14320.12851117293368033No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14310.12842143049448085No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCACCCAGACTATTTTATACAACTCCAA14080.12635735439289242No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14030.1259086421968949No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCACCTTGGTCC13970.1253701875616979No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13940.12510096024409945No Hit
GCCAAGGGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCATCTGTC13880.12456250560890245No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13530.12142152023692004No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13210.11854976218253611No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCATAAAATCGGTCAGGGACC13090.11747285291214214No Hit
ATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGA12430.11154985192497531No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12260.11002423045858387No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12250.10993448801938438No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCACCCAGACTATTTTATACAACTCCAACAATAGGAACTA12120.10876783630979091No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAGCACTATAAAATTGCTGACAGTAATAAACCGCCACATCTTCAGC11990.10760118460019744No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11880.10661401776900298No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11870.10652427532980348No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCACC11860.10643453289060398No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11560.10374225971461905No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11560.10374225971461905No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11490.10311406264022258No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11410.10239612312662658No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11390.10221663824822758No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11360.1019474109306291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA200.007679188651.1954525
GTGGTAT3250.048.2121241
GTATCAA23900.044.609591
TCGTATG503.792904E-641.47854640
TATCAAC26400.040.51242
AGAGTCG857.6397555E-1140.07401711
AACGCAG29850.036.5163546
CAACGCA29850.036.174015
TGGTATC4500.035.5752222
TCAACGC31650.034.3304334
ATCAACG31950.034.008083
ACGCAGA32300.033.9574557
TATAAGA2150.033.2692572
ATAAGAG1950.033.1880153
CGCAGAG33150.032.881248
GTATAAG2000.032.3597641
GGTATCA9200.032.2116661
GCAGAGT34000.031.9590249
CAGAGTA32950.031.9436710
AGAGTAC33100.031.69600311
Produced by FastQC (version 0.11.5)