FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114300
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38010.3411110113972898No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36800.3302521762541506No Hit
ATTTTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCAT35330.31706003769182445No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33450.30018845912231895No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32570.2922911244727632No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31750.28493224445840437No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCGGGGGTCCCT31560.28322713811361394No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGTTGGAGTTGTATAAAATAGT29910.2684196356456968No Hit
CTATAAAATTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTT28980.2600735888001436No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28620.2568428609889617No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27930.25065063268419635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27050.24275329803464057No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26950.24185587364264563No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26080.23404828143228934No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25880.23225343264829937No Hit
ATTTTATGGCAGCGGGTCTGGAACAGATTTCACTCTCACCATCAGCAGCC25180.22597146190433456No Hit
GTTTATTACTGTCAGCAATTTTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGAA23980.21520236920039487No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23140.2076640043076371No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22960.20604864040204615No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22940.20586915552364715No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22840.20497173113165215No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22440.2013820335636723No Hit
GTGCAGCCACAGTTCGTTTAATTTCCACCTTGGTCCCTTGGCCGAACGTC22100.19833079063088935No Hit
GTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA21570.19357444135331597No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21410.19213856232612403No Hit
ATTTTATACAACTCCAACAATAGGAACTACTTAGCTTGGTACCAGCAGAA21280.19097191061653054No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21180.19007448622453557No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20420.1832540608453738No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA19350.17365161985102756No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18560.16656196715426724No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCATAAAATCGGTCAGGGACC18470.16575428520147178No Hit
GGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATTTTATAGTGCTCCGT18450.16557480032307278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18030.1618056178766939No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17530.157318495916719No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17400.15615184420712555No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17330.15552364713272906No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATTTTATAGTGCT17290.1551646773759311No Hit
GCCATAAAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGTAAA17160.1539980256663376No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17140.1538185407879386No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGTTGGAGTTG16340.14663914565197883No Hit
GAGTGAAATCTGTTCCAGACCCGCTGCCATAAAATCGGTCAGGGACCCCC16060.1441263573543929No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15690.14080588710401148No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15690.14080588710401148No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15640.140357174908014No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCACCCAGACTATTTTATA15610.1400879475904155No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15490.13901103832002154No Hit
GTTCCAGACCCGCTGCCATAAAATCGGTCAGGGACCCCCGATTCCCGGGT15360.13784438661042808No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATTTTATAGTGCTCCGTGGACGTT15210.13649825002243562No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15200.1364085075832361No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15090.13542134075204165No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15070.13524185587364265No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14940.13407520416404917No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14820.13299829489365522No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14810.1329085524544557No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14610.13111370367046576No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATTTTATGGCAGCGGGTCTGGAACAGATTT14380.1290496275688773No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14360.1288701426904783No Hit
GGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC14180.1272547787848874No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14150.12698555146728888No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14150.12698555146728888No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA14050.1260881270752939No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14030.1259086421968949No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13630.12231894462891502No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13540.12151126267611954No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13320.11953692901373059No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13110.11765233779054114No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAGCACTATAAAATTGCTGACAGTAATAAACCGCCACATCTTCAGC12940.11612671632414968No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12940.11612671632414968No Hit
GTATAAAATAGTCTGGGTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA12720.11415238266176074No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCACCCAGACTATTTTATACAACTCCAA12260.11002423045858387No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG12210.10957551826258638No Hit
GCCAAGGGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCATCTGTC12060.10822938167459391No Hit
ATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGA12020.10787041191779592No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11810.10598582069460648No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11650.10454994166741452No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11530.10347303239702055No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11430.10257560800502558No Hit
CAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT11420.10248586556582608No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT11420.10248586556582608No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA11370.10203715336982858No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11370.10203715336982858No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11340.1017679260522301No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11330.1016781836130306No Hit
GGTCTGGAACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT11300.1014089562954321No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11280.10122947141703312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3500.048.6687281
GTATCAA24600.040.854031
TATCAAC27200.037.426332
GGTATCA10800.036.2762261
TGGTATC4800.035.4653052
TTAACGG400.002649219734.358335
GTTATTA1052.2046042E-433.516470
AACGCAG30600.033.3596426
CAACGCA30450.033.300485
ATCAACG31850.032.1630023
TCAACGC32000.031.9001564
ACGCAGA32600.031.208667
CGCAGAG33150.030.7935148
CAGAGTA33050.030.68077710
TAACGGC450.00470095830.56148136
AGAGTAC33200.030.54215611
TTTAACG450.004732318730.51996834
GCAGAGT33750.030.2460739
AGTACTT13300.028.14249413
CGTAGCG500.007035866428.14071754
Produced by FastQC (version 0.11.5)