FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67296
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACTT200.00680561652.72751253
CACGCAC200.006846782352.6472651
CCCCCCA200.007311942551.7803136
TTAGTAC200.007442785451.54880528
CTATACT200.007508856751.433834
GTGGTAT552.6775524E-949.875231
GTATCAA3000.040.004091
TGGTATC853.0595402E-936.3062322
TACGGGA952.2555469E-1036.09391815
CAACGCA3450.034.7861635
TATCAAC3500.034.289222
ATCAACG3500.034.289223
TCAACGC3500.034.289224
AACGCAG3550.033.806276
GAGTACG3100.033.18311712
AGTACGG3200.032.14614513
ACGCAGA3900.030.7723777
AGAGTAC4050.029.63265611
CAGAGTA4150.028.9186210
GTACGGG3450.028.82282314
Produced by FastQC (version 0.11.5)