FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117280
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT111980.35922342555047987No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCT96650.31004593748396037No Hit
GACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGAGAA77940.2500256633988605No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT71470.2292703895703947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG69810.22394523430683158No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC67280.21582918441718418No Hit
ATATTATAGTATTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT65580.21037571215931838No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65120.20890006672483705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63640.20415233793563617No Hit
GCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTAGAAAAGACT62360.20004619411794897No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG60650.1945606426115075No Hit
ATAGTATTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA60580.19433608787147771No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58650.1881447928963712No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC57400.18413488682441104No Hit
GAATACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT53990.17319586306010368No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC53960.17309962531437664No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT50780.16289842426730997No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC50780.16289842426730997No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCTGA49300.15815069547810914No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTCTA49070.15741287276086843No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT46390.14881563414258586No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA45640.14640969049940974No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC44360.14230354668172254No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG43380.13915978032130574No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42680.13691423292100804No Hit
GTTTATTACTGTCAGCAATATTATAGTATTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA39460.12658471487963865No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC39240.12587897141097368No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA38580.12376174100497869No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37520.12036134065595647No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36510.11712133654981265No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCTCGGGT36440.11689678180978287No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA36050.11564569111533131No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35130.11269440024636863No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA35130.11269440024636863No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34470.11057716984037365No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTATT34030.10916568290304368No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTCTACAGCTCCAACAATAAGAACTA32410.1039688446337833No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTCTACAGCTCCAA32290.10358389365087513No Hit
GCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT31970.10255735769645331No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31520.10111379151054765No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31420.10079299902479084No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31180.10002309705897448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10850.042.964351
GGTATCA31400.040.8263971
TGGTATC11100.040.4202272
GTATCAA77800.039.4754871
TATCAAC86400.035.160932
ATCAACG89450.033.98563
TCAACGC90400.033.5110784
CAACGCA91400.033.369145
AACGCAG94100.032.5208176
CGCAGAG101850.030.1465748
ACGCAGA103250.029.671987
AGAGTAC104350.029.42385511
CAGAGTA106950.028.77255810
ACTTGCA28700.026.5458570
GCAGAGT117550.026.1493159
CAGTTCG46900.024.15107770
AGTACGG74450.023.99967613
GAGTACT53850.023.96377612
AGTACTT54150.023.64137613
GAGTACG76750.023.41426312
Produced by FastQC (version 0.11.5)