FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684947
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76990.2867468147415945No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58090.21635436379191098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG50220.1870427982377306No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50130.18670759609035112No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49470.18424944700956852No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47560.17713571254851587No Hit
CTACAAACCCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAACG44880.1671541374932168No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44740.16663271193062656No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCCCCTGGCCAAAAGTG44550.16592506295282552No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTCCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA42660.15888581785785716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41370.15408125374541842No Hit
CTTTTGGCCAGGGGACCACGCTGGAGATCAAACGAACTGTGGCTGCACCA40790.15192106212897313No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA40120.14942566836514834No Hit
GGGTTTGTAGAACTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTCCTGATTTTCAGTGTAAAAT37000.13780532725599426No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC36960.1376563485238256No Hit
GGCCAAAAGTGTACGGGGTTTGTAGAACTTGCATGCAGTAATAAACCCCA36860.137283901693404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36080.1343788164161155No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTC35790.1332987206078928No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35600.13259107163009176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35520.13229311416575448No Hit
GGTCTAGTCAGAGCCTCCTCCATAGTAATGGATATAATAATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC33850.12607325209771364No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGGAGAG33820.12596151804858718No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCC33300.12402479453039483No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32810.12219980506132895No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31820.11851258144015506No Hit
GATTTTACACTGAAAATCAGGAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTCTACAAACC31770.11832635802494426No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31700.11806564524364913No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31240.11635238982370974No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31180.11612892172545679No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29000.10800958082226578No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28460.10599836793798909No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT27760.10339124012503785No Hit
GTTTATTACTGCATGCAAGTTCTACAAACCCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAACGAA27050.10074686762904445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92700.042.4996221
GTGGTAT9150.042.2347261
GGTATCA35250.039.7774621
TATCAAC104650.037.581932
ATCAACG107600.036.583353
TCAACGC108500.036.2798964
CAACGCA110200.035.7202265
AACGCAG112750.034.791036
CGTATGC5200.033.985641
TCGTATG5300.033.9552440
TGGTATC11850.032.9008332
CGCAGAG123700.031.7348188
ACGCAGA124550.031.4907887
ACGAGAC8200.031.29348422
GGCAATC6350.030.85397133
AGAGTAC128150.030.76567311
CAGAGTA128650.030.56636410
CGACCGG3750.030.51601470
TATGCCG5700.030.46258743
CTTATAC8650.030.4429681
Produced by FastQC (version 0.11.5)