FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684947
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75960.282910612388252No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70640.26309644100982255No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC55500.2067079908839914No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47590.17724744659764236No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47490.17687499976722074No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47080.17534796776249215No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44870.16711689281017464No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCCCCTGGCCAAAAGTG43830.16324344577378996No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTCCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA43620.16246130742990456No Hit
GGGTTTGTAGAACTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTCCTGATTTTCAGTGTAAAAT42680.15896030722394147No Hit
CTACAAACCCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAACG40640.15136239188334072No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39080.14555222132876366No Hit
CTTTTGGCCAGGGGACCACGCTGGAGATCAAACGAACTGTGGCTGCACCA38670.14402518932403507No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37720.14048694443502982No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37000.13780532725599426No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA36980.13773083788990992No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35380.13177168860316424No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34470.1283824224463276No Hit
GGCCAAAAGTGTACGGGGTTTGTAGAACTTGCATGCAGTAATAAACCCCA34300.12774926283461088No Hit
GGTCTAGTCAGAGCCTCCTCCATAGTAATGGATATAATAATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC34170.1272650819550628No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTC33920.12633396487900878No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGGAGAG33320.12409928389647915No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC32630.12152940076657008No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32140.1197044112975042No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31230.11631514514066758No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31050.1156447408459087No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCC30320.11292587898383097No Hit
GATTTTACACTGAAAATCAGGAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTCTACAAACC30140.11225547468907207No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29850.11117537888084943No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29850.11117537888084943No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28440.10592387857190477No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT27760.10339124012503785No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27750.1033539954419957No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27060.10078411231208662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)