FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670450
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29570.1770181687569218No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25360.1518153790894669No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21230.12709150228980215No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20950.12541530725253675No Hit
GTCCCAGACCCACTGCCACTGAACCTGGGTGGGATACCAGCGGCCCTGGT19120.11446017540183782No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18570.11116764943578078No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18280.10943159029004161No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18100.10835403633751384No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18030.1079349875781975No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCACCAGTGGTTACTTCTGGACCTGGATCCGCCAGCACCCAGGGAAGGG17750.10625879254093208No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17510.10482205393756173No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17410.10422341285282409No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGAAG17290.10350504355113892No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17220.10308599479182255No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGAAGCAACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT16880.10105061510371456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30950.041.053421
TATCAAC34700.036.5181122
CTCGTAT1350.035.78756739
TCGTATG1550.035.6473840
ATCAACG36600.034.8105553
TCAACGC37050.034.2953154
CAACGCA37200.034.2490965
AACGCAG38400.033.446386
GGTATCA13200.032.4318851
AGAGTAC41850.030.19360711
CGCAGAG42800.029.8443538
GCAGAGT44900.028.5247849
ACGCAGA45100.028.3982877
CAGAGTA46900.026.86946910
TCTATAC1306.8212103E-926.3454573
TATGCCG2100.024.73840343
AGTACGG36350.024.39941613
GAGTACG37100.023.72156312
CGTATGC2250.023.04870641
TATACAC3000.022.832733
Produced by FastQC (version 0.11.5)