FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670450
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29250.17510251728576132No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24300.14546978359124785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22560.13505342871681283No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22460.1344547876320752No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21340.12775000748301354No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21050.1260139483372744No Hit
GTCCCAGACCCACTGCCACTGAACCTGGGTGGGATACCAGCGGCCCTGGT19330.11571732167978688No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18870.11296357268999371No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18770.11236493160525607No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCACCAGTGGTTACTTCTGGACCTGGATCCGCCAGCACCCAGGGAAGGG17070.10218803316471609No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16900.1011703433206621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)