FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494252
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACTACTGTCAACATTATGGTACCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA28570.19119934254730797No Hit
GTAGTACACTGCACAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23860.15967855488900132No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22800.15258470458798112No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15610.10446698414992919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.036.0582281
GTATCAA28800.035.557421
TATCAAC31700.031.9793172
TCAACGC33150.030.6841874
CTACGCT1800.030.57052428
ATCAACG33400.030.3516273
CAACGCA34200.029.6416475
AACGCAG35700.028.5887176
AGAGTAC39100.026.01224311
ACGCAGA40150.025.5887157
CGCAGAG40600.025.3897298
CAGAGTA40450.025.31398810
GCAGAGT41800.024.5786349
ACTACGC2100.024.55984127
GTGGTAT7450.023.0632321
CAGTTCG9600.022.70743470
TACGCTG2450.022.46298429
TTCGGGG8350.022.15118470
CTTATAC3000.021.7640041
GTACACT11200.021.170814
Produced by FastQC (version 0.11.5)