FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991906
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43460.4381463566104046No Hit
GTTAGGACCCGGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC34130.34408502418575954No Hit
GTATAATAATTGGCGAGGCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCAT29440.296802317961581No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT28930.2916607017197194No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28690.28924111760590215No Hit
CATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA27900.2812766532312538No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT27690.27915951713166365No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27570.27794972507475507No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27170.27391708488505967No Hit
TTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC27060.2728081088328934No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25160.25365306793184034No Hit
TCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT24160.2435714674576018No Hit
CTCCTGTAGGGCCAGTCAGAGTGTGCACAGCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT23840.24034535530584553No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGCCTC22920.23107028286954612No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22730.2291547787794408No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATAATAATTGG22590.22774335471304738No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22230.22411397854232157No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT22180.2236098985186096No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGAC22150.22330745050438247No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG21840.22018215435736851No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21150.21322585003014397No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTGCACAG20770.20939484184993337No Hit
GTGTAAGGATCACCTGGTTTTTGGAGGCGTCCTTGGTGATGTTGAGTCTG20520.20687444173137373No Hit
GAGTGTGCACAGCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTC20310.20475730563178368No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19200.19356672910537895No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18550.1870136887971239No Hit
TCCTTACACTGTCCAACGTGGACCCTGGGGACACAGCCATATATTTCTGTGCACACGCTCCATACGTGGATACAA18460.18610634475444246No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18200.18348512863114044No Hit
GCTCAGTTAGGACCCGGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT17620.17763780035608212No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA17600.17743616834659737No Hit
CTGCTACTCTGCCTCCCAGTTTCAGATACCACTGGAGAAATCTTGATGAC16200.1633219276826635No Hit
GTCGTGTCCCTTGGCCGAAGGTGCCTCGCCAATTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGA16130.1626162156494668No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16110.162414583639982No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15940.16070071155936147No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15820.15949091950245284No Hit
GTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTGCACAGCTATTTAGTCTGGTA15570.15697051938389323No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC15280.15404685524636408No Hit
GTAATAAACTGCAGAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA15160.15283706318945545No Hit
GTTTATTACTGTCAGCAGTATAATAATTGGCGAGGCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAA15160.15283706318945545No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14850.14971176704244152No Hit
CACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGACTAAATAGCTGTGCACACTCTGA14790.14910687101398723No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14170.14285627871995935No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14080.14194893467727787No Hit
GTCAGAGTGTGCACAGCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG13490.1360007903974772No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13210.1331779422646904No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13060.13166570219355464No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12840.12944775008922216No Hit
GGATACCAGTGGCCCTGGTGGACGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGA12800.12904448607025262No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12640.12743142999437446No Hit
CGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT12320.12420531784261815No Hit
GCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTCCACCAGGGCCA12250.12349960580942144No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12210.12309634179045192No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11810.11906370160075654No Hit
CCTGTAGGGCCAGTCAGAGTGTGCACAGCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTC11790.11886206959127174No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11610.11704738150590883No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11510.11603922145848498No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11360.11452698138734921No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11030.11120005323085051No Hit
CTCCCAGGCTCCTCATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCA10720.10807475708383657No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10670.10757067706012464No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10440.1052519089510498No Hit
CCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCA10290.10373966887991402No Hit
CCTCATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT10140.10222742880877825No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9980.1006143727329001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)