FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991906
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43830.4418765487858729No Hit
GTTAGGACCCGGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC36530.3682808653239319No Hit
GTATAATAATTGGCGAGGCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCAT32340.32603895933687266No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT29030.29266886176714324No Hit
TTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC28350.28581337344466107No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT28290.28520847741620675No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27660.2788570691174365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25680.2588955001784443No Hit
CATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA25530.25738326010730855No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25410.2561734680503999No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATAATAATTGG23610.23802658719677067No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG23200.2338931310023329No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23090.23278415495016666No Hit
CTCCTGTAGGGCCAGTCAGAGTGTGCACAGCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT22520.22703764267985072No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGCCTC22100.22280337048067056No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT22020.22199684244273146No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21980.22159357842376193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21850.22028297036211092No Hit
TCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT21370.21544380213447645No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20440.20606791369343463No Hit
GTGTAAGGATCACCTGGTTTTTGGAGGCGTCCTTGGTGATGTTGAGTCTG19770.19931324137569487No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19630.19790181730930145No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19190.19346591310063654No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGAC18820.18973572092516833No Hit
GAGTGTGCACAGCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTC18260.18409002465959476No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18230.1837875766453676No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTGCACAG18160.1830818646121709No Hit
GCTCAGTTAGGACCCGGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT17900.18046064848886892No Hit
TCCTTACACTGTCCAACGTGGACCCTGGGGACACAGCCATATATTTCTGTGCACACGCTCCATACGTGGATACAA17780.1792508564319603No Hit
GTCGTGTCCCTTGGCCGAAGGTGCCTCGCCAATTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGA17640.1778394323655669No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17420.17562148026123442No Hit
CTGCTACTCTGCCTCCCAGTTTCAGATACCACTGGAGAAATCTTGATGAC17380.17521821624226488No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17170.1731010801426748No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA16610.16745538387710127No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC16190.1632211116779211No Hit
GTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTGCACAGCTATTTAGTCTGGTA15530.1565672553649237No Hit
GTTTATTACTGTCAGCAGTATAATAATTGGCGAGGCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAA15120.1524337991704859No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15090.15213135115625875No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14950.15071992708986537No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14840.14961095103769914No Hit
GTAATAAACTGCAGAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA14440.14557831084800374No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14060.14174730266779312No Hit
CACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGACTAAATAGCTGTGCACACTCTGA13690.13801711049232487No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13520.13630323841170433No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13240.13348039027891756No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13020.1312624381745851No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12930.13035509413190363No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12890.12995183011293407No Hit
GTCAGAGTGTGCACAGCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG12820.1292461180797374No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12800.12904448607025262No Hit
GGATACCAGTGGCCCTGGTGGACGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGA12770.1287420380560255No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12030.12128165370508899No Hit
CGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT11640.11734982952013596No Hit
GCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTCCACCAGGGCCA11590.11684574949642405No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11480.11573677344425783No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11330.11422453337312205No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10740.10827638909332135No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10710.1079739410790942No Hit
CCTGTAGGGCCAGTCAGAGTGTGCACAGCTATTTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTC10580.10666333301744318No Hit
CTCCCAGGCTCCTCATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCA10410.10494946093682264No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10360.10444538091311073No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTGCCTCGCCAATT10340.10424374890362594No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10330.10414293289888357No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10210.10293314084197495No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10040.10121926876135441No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG10010.10091682074712725No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9980.1006143727329001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20600.039.9630051
GGTATCA8750.035.6736451
TATCAAC23800.034.69192
TAACACG400.002696529534.2341464
TCAACGC24350.033.6014824
ATCAACG24750.032.921773
CAACGCA25200.032.46815
AACGCAG25250.032.403816
GTCTATA450.004754652330.4902951
CGCAGAG27000.030.0499738
AGAGTAC27850.029.1328311
ACGCAGA28350.028.8605337
AATGGTA1505.993982E-527.70856770
TAGCTCT5700.027.2612669
GGATGTA5500.027.05763664
TGTTAGA7000.026.71897770
ATGGGAT6050.026.20989261
CAGAGTA31350.025.98956110
GGGATGT6300.025.80680863
ATGTAGC5550.025.80630166
Produced by FastQC (version 0.11.5)