FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684863
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA50360.18757009203076658No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50290.18730937109267773No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36820.13713921343472646No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGCC35750.13315390766679716No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG33700.12551850876562418No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32340.12045307339704113No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30030.1118492824401096No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28460.10600168425726005No Hit
GTGCAGCCACAGTTCGTTTGATATCCACCTTGGTCCCTTGGCCGAACGTC28310.10544299653278398No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28100.10466083371851748No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28000.10428837523553344No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27670.10305926224168607No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27190.10127146152336265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53650.038.696011
GGTATCA23350.038.5667761
TATCAAC61400.033.586572
TCAACGC64000.032.2239154
GTGGTAT8750.032.211041
ATCAACG64250.032.0438423
CAACGCA65300.031.5309375
AACGCAG67150.030.6628236
TGGTATC9550.028.0720422
ACGCAGA73800.027.8993247
CGCAGAG76300.026.9851918
CGATTGT1457.6584045E-526.73410470
AGAGTAC77000.026.55982611
CAGAGTA80000.025.8225810
GCAGAGT81700.025.285749
AATGGTA11650.023.84651670
TGACTTG26450.022.47214770
ACAGTCG14100.021.9405069
CGACCGG12800.021.75073811
AGTACGG55100.021.27048913
Produced by FastQC (version 0.11.5)