FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684863
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52450.19535447432513317No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA47050.17524171624399457No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35290.13144059864507052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32600.12142146545279964No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31280.1165050134774102No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31050.1156483589665469No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGCC30770.11460547521419157No Hit
GTGCAGCCACAGTTCGTTTGATATCCACCTTGGTCCCTTGGCCGAACGTC29330.10924207305922128No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28120.10473532541511428No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27920.1039904084491462No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27300.1016811658546451No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC26850.10000510268121689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9450.040.7311251
GTATCAA59400.037.7804341
GGTATCA26250.037.3126221
TATCAAC65600.033.9205742
ATCAACG68000.032.5050283
TCAACGC67850.032.4690634
TGGTATC11800.031.7202452
CAACGCA69550.031.6260875
AACGCAG70500.031.443296
ACGCAGA74700.029.6294577
CGCAGAG76800.028.7745958
AGAGTAC77500.028.38186311
CAGAGTA79850.027.58955410
GCAGAGT82150.027.025979
AATGGTA12800.023.7352570
AGTACGG57400.023.67379213
GAGTACG58700.023.32487712
GAGTACT37850.022.21185112
ACAGTCG12700.021.345589
GTACTAG1305.093585E-621.1488531
Produced by FastQC (version 0.11.5)